PXD074038

PXD074038 is an original dataset announced via ProteomeXchange.

Title	Mass Spectrometric Profiling of hepatic GSTM3 - co-immunoprecipitate in Cholesterol-induced Steatohepatic Apoe knockout Mice
Description	Glutathione S-transferase Mu 3 (GSTM3) is a cytosolic detoxification isoform of the glutathione S-transferase family, implicated in cellular redox homeostasis and protection against lipid peroxidation–derived reactive products. Emerging evidence suggests that GSTM3 functions beyond xenobiotic metabolism and may contribute to the pathogenesis of stress-induced diseases, including cardiovascular disorders, neurodegenerative diseases, and cancer. However, how the GSTM3-associated interactome links oxidative stress responses to metabolic and inflammatory signaling pathways remains largely unknown. In this study, we performed mass spectrometry–based proteomic profiling of hepatic GSTM3-associated protein complexes using co-immunoprecipitation followed by LC–MS/MS analysis. GSTM3-containing complexes were isolated from the livers of control and cholesterol-induced steatohepatitis Apoe⁻/⁻ mice. The resulting datasets provide an unbiased characterization of the GSTM3 interactome under both basal and disease-associated conditions, enabling the identification of proteins potentially involved in redox regulation, metabolic stress responses, and downstream inflammatory signaling pathways.
HostingRepository	PRIDE
AnnounceDate	2026-05-20
AnnouncementXML	Submission_2026-05-20_03:48:01.220.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD074038
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Trina Roy
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-04 00:31:56	ID requested
⏵ 1	2026-05-20 03:48:01	announced

10.6019/PXD074038;

10.1016/J.ATHEROSCLEROSIS.2026.120764;

submitter keyword: ApoE null mice, cholesterol-induced steatohepatitis, liver, Glutathione-s-transferase Mu 3, Orbitrap, Co-immunoprecipitation

Dr. Arun Bandyopadhyay
contact affiliation	Department of Animal Biotechnology, Gujarat Biotechnology University, Gandhinagar 382355, India; former Professor, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad - 201002, India. Former Director, CSIR-Indian Institute of Chemical Biology, IICB, TRUE Campus, CN-6, Sector 5, Salt Lake, Kolkata 700091, WB, India
contact email	arunb2523@gmail.com
lab head
Trina Roy
contact affiliation	CSIR-SRF, CSIR - Indian Institute of Chemical Biology
contact email	animetrina18@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074038
     1. Label: PRIDE project
     2. Name: Mass Spectrometric Profiling of hepatic GSTM3 - co-immunoprecipitate in Cholesterol-induced Steatohepatic Apoe knockout Mice