PXD074013

PXD074013 is an original dataset announced via ProteomeXchange.

Title	A global S-palmitoylation map of Trypanosoma cruzi identifies ABCA10 as a key component of a redundant heme-acquisition system
Description	The survival and continued transmission of the human infecting parasite Trypanosoma cruzi depends on the dynamic remodeling of its membrane proteome, yet the post-translational networks governing this plasticity remain largely obscure. Here, we deployed metabolic labeling coupled with quantitative mass spectrometry to generate the first comprehensive S-palmitoylome of T. cruzi. We defined a high-confidence atlas of 254 S-palmitoylated proteins, revealing an enrichment in signaling factors, cytoskeletal regulators, and membrane transporters. Leveraging our aptazyme-based SHARK conditional knockdown system for scalable genetic screening, we validated palmitoylated candidates and uncovered a critical link between lipid signaling and nutrient acquisition. Our functional screen identified ABCA10, a previously uncharacterized ATP-binding cassette (ABC)-transporter, as central to heme homeostasis. Strikingly, depletion of ABCA10 triggered a massive compensatory surge in cytostome-mediated endocytosis, rendering this normally dispensable pathway conditionally essential for survival. We further demonstrate that ABCA10 is a heme-responsive transporter that dynamically redistributes from the flagellar pocket to lysosomal compartments upon heme starvation, exposing a redundant, multi-modal strategy for scavenging this essential cofactor. This study not only provides a foundational proteomic resource but also exposes a novel, druggable vulnerability at the intersection of lipid signaling and metabolic survival.
HostingRepository	PRIDE
AnnounceDate	2026-07-30
AnnouncementXML	Submission_2026-07-30_11:57:23.196.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD074013
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sophe Alvarez
SpeciesList	scientific name: Trypanosoma cruzi; NCBI TaxID: NEWT:5693;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-03 16:31:33	ID requested
⏵ 1	2026-07-30 11:57:23	announced

10.6019/PXD074013;

submitter keyword: Eclipse orbitrap, S-palmitoylome, membrane proteome,Trypanosoma cruzi

Sophie Alvarez
contact affiliation	Proteomics and Metabolomics Facility, University of Nebraska - Lincoln
contact email	salvarez@unl.edu
lab head
Sophe Alvarez
contact affiliation	University of Nebraska - Lincoln
contact email	salvarez@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD074013

PRIDE project URI

• PRIDE
  1. PXD074013
     1. Label: PRIDE project
     2. Name: A global S-palmitoylation map of Trypanosoma cruzi identifies ABCA10 as a key component of a redundant heme-acquisition system