PXD074007

PXD074007 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of WT and KO for DPP3 Mus musculus splenocites, treated (and not) with LPS
Description	Effective host defense against infection relies on the tight coordination of immune activation, metabolic adaptation, and redox control, yet how these processes are integrated remains incompletely understood. Here, we identify dipeptidyl peptidase 3 (Dpp3) as a negative regulator of antimicrobial immunity. Dpp3-/- mice exhibit enhanced resistance to Klebsiella pneumoniae infection, with reduced bacterial burden, preserved tissue integrity, and attenuated systemic inflammation. This response is associated with increased phagocytic activity, expansion of germinal centres and plasma cells, and elevated interferon-γ production by T cells. Mechanistically, Dpp3 deficiency leads to reduced Nrf2 protein levels upon stimulation, resulting in heightened ROS accumulation and amplified NF-κB signaling. Integrated metabolomic and transcriptomic analyses of Dpp3-/- immune cells reveal mitochondrial dysfunction and a shift toward biosynthetic and antioxidant-supportive metabolic programs. Collectively, our findings identify Dpp3 as a molecular brake on host defense and uncover a regulatory axis linking redox balance, immunometabolism, and inflammation during infection.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_00:26:37.139.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raffaello Viganò
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-03 14:38:39	ID requested
⏵ 1	2026-05-21 00:26:37	announced

Dataset with its publication pending

submitter keyword: Mus musculus, Immunity,Splenocites

Dario Di Silvestre
contact affiliation	Institute for Biomedical Technologies, National Research Council (ITB-CNR)
contact email	dario.disilvestre@itb.cnr.it
lab head
Raffaello Viganò
contact affiliation	Institute for Biomedical Technologies, National Research Council (ITB-CNR)
contact email	raffaello.vigano@itb.cnr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074007
     1. Label: PRIDE project
     2. Name: Proteomic profiling of WT and KO for DPP3 Mus musculus splenocites, treated (and not) with LPS