PXD073998

PXD073998 is an original dataset announced via ProteomeXchange.

Title	Human Lactyl Adenosine IPRP-UHPLC QTOF-MS
Description	Dynamic coordination of energy metabolism and protein synthesis is pivotal to cell fitness, especially in cancer cells facing microenvironmental challenges. Whether this rapid communication depends on complex signaling pathways or can be mediated by a single molecule remains unclear. Here, we show that intracellular accumulation of lactate, a central glycolytic product, is sufficient to acutely repress mRNA translation. Mechanistically, lactate is charged to tRNA aminoacylation sites instead of amino acids by AARS1. The slower charging kinetic of lactate extends lactylation to non-cognate tRNAs, further exacerbating translation repression. This direct metabolic control over the central dogma may function to restrict protein synthesis during metabolic imbalance, yet, cancer cells evade this brake by enhancing lactate export to sustain pro-tumor translation. Pharmacological blockade of lactate export re-establishes translational repression and impairs tumor progression. These findings uncover a unique metabolite-tRNA charging event directly rewiring translational output and reveal a metabolic vulnerability with therapeutic potential.
HostingRepository	PRIDE
AnnounceDate	2026-09-04
AnnouncementXML	Submission_2026-09-04_01:21:07.157.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	yichen xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	lactic acid
Instrument	Vion IMS QTof

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-03 12:00:17	ID requested
⏵ 1	2026-09-04 01:21:07	announced

Wang X, Lin C, Zhang Y, Wang H, Shi B, Bu Z, Xu P, Zheng H, Wang Y, Lan X, Hu Q, Huang M, Wang Y, Tang H, Xu Y, Excess intracellular lactate represses mRNA translation and tumor progression through lactate-tRNA charging. Mol Cell, 86(17):3529-3544.e9(2026) [pubmed]

10.1016/j.molcel.2026.07.026;

submitter keyword: Lactyl-Adenosine

UHPLC QTOFMS

yichen xu
contact affiliation	Zhejiang University College of Pharmaceutical Sciences
contact email	yichen_xu@zju.edu.cn
lab head
yichen xu
contact affiliation	Zhejiang university
contact email	yichen_xu@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD073998

PRIDE project URI

• PRIDE
  1. PXD073998
     1. Label: PRIDE project
     2. Name: Human Lactyl Adenosine IPRP-UHPLC QTOF-MS