PXD073977

PXD073977 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in Murine Asthma Model
Description	Extracellular vesicles (EVs) are increasingly recognized as key mediators of intercellular communication and immune regulation. However, their proteomic composition in allergic asthma remains poorly understood. In this study, we performed tandem mass tag (TMT)-based quantitative proteomic analyses of the whole bronchoalveolar lavage fluid (BALF) supernatant and EVs enriched from BALF in a murine model of house dust mite (HDM)-induced asthma. Using LC-MS/MS analysis in the data-dependent acquisition (DDA) mode, we identified more than 400 proteins across all samples, revealing distinct enrichment of canonical EV markers in EV fractions. HDM challenge induced significant upregulation of Th2-associated proteins, e.g., CLCA1, FCGBP, CHIL3, CHIL4, and RETNLA, consistent with hallmark features of asthma. Notably, proteins such as EPX and CKM were detected exclusively in EVs, not in whole BALF, underscoring the potential of EVs to selectively carry disease-relevant proteins. Our results provided insights into the distinct proteomic profiles of EV-associated versus BALF proteins, highlighting potential EV-mediated mechanisms in asthma pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2026-04-20
AnnouncementXML	Submission_2026-04-20_13:20:27.948.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zachary Hutchins
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-02 21:30:16	ID requested
⏵ 1	2026-04-20 13:20:28	announced

10.1021/ACS.CHEMRESTOX.6C00094;

submitter keyword: ultracentrifugation, mouse model, asthma, TMT, mass spectrometry,Extracellular vesicles, HDM, bronchoalveolar lavage fluid

Yinsheng Wang
contact affiliation	Department of Chemistry, University of California, Riverside, CA 92502, USA
contact email	yinsheng@ucr.edu
lab head
Zachary Hutchins
contact affiliation	University of California, Riverside
contact email	zachary.hutchins@email.ucr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073977
     1. Label: PRIDE project
     2. Name: Quantitative Proteomic Profiling of Bronchoalveolar Lavage-Derived Extracellular Vesicles in Murine Asthma Model