PXD073886

PXD073886 is an original dataset announced via ProteomeXchange.

Title	Proteomics Identification of Opisthorchis viverrini Antigens in Excretory-Secretory matrix
Description	Opisthorchis viverrini (Ov), a Group I biological carcinogen classified by the WHO, is a leading cause of cholangiocarcinoma (CCA) in the Greater Mekong Subregion. Transmission occurs via consumption of raw or undercooked fish harboring Ov metacercariae, which mature into adult Ov worms in the biliary tract, triggering chronic inflammation and malignant transformation. Despite the widespread use of Ov-antigen rapid test kits (Ov-ATKs) for non-invasive screening, current diagnostics are limited by cross-reactivity and dependence on crude Ov antigens. In this study, we employed a label-free proteomics approach to comprehensively analyze infection-associated stages of Ov (metacercariae and adult worms) to compare with urinary sample from Ov-infected and CCA patients. A total of 17,287 proteins were identified, 2,768 protein shares across group of samples and 3,977 proteins were consistently upregulated in Ov-positive urine samples, we provide the first direct evidence that Ov-derived proteins are present in the urine of infected individuals, confirming systemic dissemination from the parasite. We also identified key protein signatures associated with both acute and chronic infection. These findings offer key insights into parasite specific protein and support the development of recombinant antigen-based diagnostics, enhancing Ov-ATKs specificity and enabling more accurate, scalable screening in endemic areas.
HostingRepository	PRIDE
AnnounceDate	2026-02-26
AnnouncementXML	Submission_2026-02-26_00:26:44.317.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuthamat Klanghon
SpeciesList	scientific name: Opisthorchiidae; NCBI TaxID: NEWT:6196;
ModificationList	monohydroxylated residue
Instrument	compact

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-01 01:56:00	ID requested
⏵ 1	2026-02-26 00:26:44	announced

Dataset with its publication pending

submitter keyword: Antigens, Opisthorchis viverrini (Ov), Excretory-secretory maxtrix, Proteomics, Urine-Based Diagnostics

Integrative Omics Medicine & Synthetic Biology Research Group
contact affiliation	Khon Kaen University National Phenome Institute, Regional Science Park Northeast 1, Khon Kaen University, Khon Kaen, 40002, Thailand
contact email	jutarop@kku.ac.th
lab head
Chuthamat Klanghon
contact affiliation	Department of Systems Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
contact email	chuthamat.kl@kkumail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD073886

PRIDE project URI

• PRIDE
  1. PXD073886
     1. Label: PRIDE project
     2. Name: Proteomics Identification of Opisthorchis viverrini Antigens in Excretory-Secretory matrix