PXD073871

PXD073871 is an original dataset announced via ProteomeXchange.

Title	Laboratory and in-situ insights into the chemoautotrophic and zero-valent sulfur formation pathway in a cold-seep bacterium
Description	Microbial sulfur cycling and chemoautotrophy are focal points of research in cold seeps. However, limited culture-dependent and in-situ studies have described the biological features and ecological significance of chemoautotrophic sulfur-oxidizing bacteria. In this study, we isolated Guyparkeria hydrothermalis SP2, a thiosulfate/sulfide-oxidizing chemoautotrophic bacterium, from cold-seep sediment. Electron microscopy, Raman spectroscopy, and stoichiometry confirmed the efficient production of zero-valent sulfur (ZVS) by G. hydrothermalis SP2. Genomic, transcriptomic, and qRT-PCR analyses revealed its utilization of the Sox pathway for thiosulfate oxidation and the fccB gene for sulfide oxidation. Its chemoautotrophic capability mediated by the Calvin-Benson-Bassham (CBB) cycle was identified through isotopic and qRT-PCR analyses. In-situ studies demonstrated its ability to produce ZVS by oxidizing sulfide in cold seeps, with a preference for different genes compared to those under laboratory conditions. Metagenomic and metatranscriptomic analyses indicated the ubiquity of its sulfur oxidation-based chemoautotrophic pathway in cold seep sediments. Therefore, this strain holds significance for investigating sulfur oxidation-based chemoautotrophic pathways in cold seeps.
HostingRepository	PRIDE
AnnounceDate	2026-02-25
AnnouncementXML	Submission_2026-02-25_06:26:05.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ruining Cai
SpeciesList	scientific name: Guyparkeria hydrothermalis; NCBI TaxID: NEWT:923;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-31 02:08:49	ID requested
⏵ 1	2026-02-25 06:26:05	announced

Dataset with its publication pending

submitter keyword: Cold seep

zero-valent sulfur

chemoautotrophy

Ruining Cai
contact affiliation	Institute of Oceanology Chinese Academy of Sciences
contact email	RuiningCAI@163.com
lab head
Ruining Cai
contact affiliation	Institute of Oceanology Chinese Academy of Sciences
contact email	1651309629@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD073871

PRIDE project URI

• PRIDE
  1. PXD073871
     1. Label: PRIDE project
     2. Name: Laboratory and in-situ insights into the chemoautotrophic and zero-valent sulfur formation pathway in a cold-seep bacterium