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PXD073850

PXD073850 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleROME BioID2 raw proteomics data from ROME KO manuscript
DescriptionFor proteomics, samples were heated for 10 min at 95 degrees. After cooling down to room temperature, 4 uL of 500 mM IAA in 5%SDS was added to a final concentration of 40 mM and incubated in the dark for 30 min. Undissolved matter was centrifuged for 8 min at 13,000 x g. The supernatant was saved and used for downstream processing using a S-Trap column (ProtiFi, LLC). Proteins were digested with sequencing-grade Lys-C/trypsin (Promega) by incubation at 37 degrees overnight. Peptides were analyzed with a nanoAcquity UPLC system coupled with Orbitrap Fusion Lumos mass spectrometer (ThermoFisher) as previously described.
HostingRepositoryMassIVE
AnnounceDate2026-03-05
AnnouncementXMLSubmission_2026-03-05_09:00:28.405.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnna Molotkova
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentnanoACQUITY UPLC; Orbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02026-01-30 10:01:12ID requested
12026-03-05 09:00:28announced
Publication List
no publication
Keyword List
submitter keyword: BioID2, ROME, proteomics, DatasetType:Proteomics
Contact List
Aykut Uren
contact affiliationGeorgetown University Medical Center
contact emailau26@georgetown.edu
lab head
Anna Molotkova
contact affiliationGeorgetown University Medical Center
contact emailam3599@georgetown.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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