PXD073836

PXD073836 is an original dataset announced via ProteomeXchange.

Title	A multi-omics approach reveals specific oncogenic and inflammatory potential of viral oncoproteins Tax from neglected HTLV-1b and -1c genotypes
Description	Over the recent years, there has been a renewed interest in the genetic variability of the human oncogenic retrovirus HTLV-1 (Human T-cell Leukemia Virus type 1), following reports of high prevalences of infection with HTLV-1 genotype c in remote aboriginal populations in Central Australia, and acknowledgment of the remarkably high genetic diversity of HTLV-1 in Central Africa. While clinical and epidemiological studies suggest that HTLV-1 genotypes might be associated with varying risks of pathological manifestations, molecular comparisons of the viral determinants among genotypes remain scarce. In this study, we provide the first comprehensive comparative analysis of the major oncoprotein Tax1 from genotypes a (Japanese), b (African) and c (Australo-Melanesia). Using unbiased analysis of image cytometry data from Tax1-expressing Jurkat T-cells, combined with proximity interactomics, we first show that Tax1 variants exhibit distinct subcellular localization in T-cells. Indeed, in contrast to Tax1a that assembles a NF-kB-activating signalosome at the surface of the Golgi apparatus, Tax1c lacks any significant association with this cell compartment. Surprisingly however, Tax1c does not show any general defect in NF-kB activation compared to Tax1a and Tax1b. Instead, transcriptomics analysis combined with pathway inference indicate that the quality of the NF-kB signature induced by Tax1c differs from that of Tax1a, and that the transcriptional landscape of Tax1c-expressing cells is biased towards T-cell activation and inflammation, an observation that is consistent with the kinome profiling of Tax1c-expressing cells. Analysis of Tax1b-induced transcriptional modulation revealed that it closely parallels that of Tax1a, but with an overall higher magnitude. In addition, distinct modulation of cell cycle-related kinase activity by Tax1b compared to Tax1a was correlated with a specific modulation of the cell cycle. Consistently, functional cell transformation assays demonstrated that Tax1b presents a higher oncogenic potential compared to Tax1a, while Tax1c harbours a decreased transforming activity. Altogether, we provide evidence of functional divergence among Tax1 from different genotypes that may have pathophysiological and clinical implications.
HostingRepository	MassIVE
AnnounceDate	2026-08-16
AnnouncementXML	Submission_2026-08-16_23:53:43.944.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Page
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: HTLV-1 subtype A; NCBI TaxID: 402042; scientific name: HTLV-1 subtype B; NCBI TaxID: 402043; scientific name: HTLV-1 subtype C; NCBI TaxID: 402044;
ModificationList	Oxidation; Biotin
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-30 02:01:21	ID requested
⏵ 1	2026-08-16 23:53:44	announced

no publication

submitter keyword: HTLV, Tax, BioID2, proximity interactomics, DatasetType:Proteomics

Chloe Journo
contact affiliation	ENS de Lyon
contact email	chloe.journo@ens-lyon.fr
lab head
Page
contact affiliation	CNRS
contact email	adeline.page@ibcp.fr
dataset submitter

MassIVE dataset URI

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