PXD073814

PXD073814 is an original dataset announced via ProteomeXchange.

Title	NFIC condensates partition NMNAT1 to limit nuclear NAD+ synthesis and promote cellular senescence
Description	This project investigates the role of Nuclear Factor I C (NFIC) in nuclear proteostasis, cellular aging, and domain-specific protein interaction networks using quantitative proteomics based on 4D Fast Data-Independent Acquisition (4D Fast DIA) mass spectrometry. For global nuclear proteome profiling, human lung fibroblast IMR-90 cells at different cellular states were analyzed. Samples NFIC01–NFIC06 represent nuclear proteomes quantified by 4D Fast DIA proteomics. Among them, NFIC01–NFIC03 correspond to the early-passage (EP, young) group, while NFIC04–NFIC06 correspond to the late-passage (LP, senescent) group. These datasets were generated to characterize age-associated alterations in the nuclear proteome and to explore NFIC-related molecular changes during cellular senescence. To further dissect the functional domains of NFIC and define its protein interaction landscape, immunoprecipitation (IP)–associated proteomic analysis was performed in HEK293T cells. Samples NFIC07–NFIC09 represent IP-derived proteomic datasets from cells expressing Flag-tagged eGFP (control), eGFP-NFIC (full-length), and eGFP-NFICΔIDR2 (IDR2 deletion mutant), respectively. These experiments were designed to compare the interactomes of full-length NFIC and its intrinsically disordered region 2 (IDR2) deletion variant, thereby defining the contribution of IDR2 to NFIC-mediated protein interactions.
HostingRepository	iProX
AnnounceDate	2026-01-29
AnnouncementXML	Submission_2026-01-29_19:42:06.148.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guowen Luo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-29 19:41:45	ID requested
⏵ 1	2026-01-29 19:42:06	announced

Dataset with its publication pending

submitter keyword: NFIC, nuclear proteostasis, cellular aging

Chenchen Zhou
contact affiliation	State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University
contact email	chenchenzhou5510@scu.edu.cn
lab head
Guowen Luo
contact affiliation	State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University
contact email	luoguowendono@163.com
dataset submitter

iProX dataset URI