⮝ Full datasets listing
PXD073807
PXD073807 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Distinct Modes of Mediator-Dependent Transcription at Heat Shock Protein Genes and Nuclear Stress Bodies |
| Description | Cells adapt to environmental stress by reprogramming gene expression. During heat stress, the transcription factor HSF1 translocates into the nucleus and activates heat shock–responsive genes, including the lncRNA HSATIII, thereby promoting formation of nuclear stress bodies (nSBs). We show that the Mediator subunit MED26 enhances HSATIII transcription and nSB formation through a mechanism distinct from its role at canonical heat shock protein (HSP) genes. Upon heat stress, MED26-containing Mediator is recruited to both HSP loci and nSBs through interactions with HSF1 and MED15. Notably, phosphorylation of the intrinsically disordered region (IDR) of HSF1 is particularly important for Mediator recruitment to nSBs. Acute loss of MED26 leads to dissociation of both Mediator and Pol II from HSP promoters, whereas at HSATIII loci Mediator is retained despite Pol II dissociation. These results indicate that MED26 acts as molecular switch that facilitates Mediator-Pol II holo-complex assembly at nSBs and promotes nSB formation during heat stress. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-07-17 |
| AnnouncementXML | Submission_2026-07-16_17:40:21.685.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hidehisa Takahashi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-29 18:10:24 | ID requested | |
| ⏵ 1 | 2026-07-16 17:40:22 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: transcription, HSF1, MED26, Mediator complex, nuclear bodies |
Contact List
| Hidehisa Takahashi | |
|---|---|
| lab head | |
| Hidehisa Takahashi | |
| contact affiliation | Yokohama city university |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004360/ |
