⮝ Full datasets listing
PXD073757
PXD073757 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A phosphorylation-dependent ubiquitination switch orchestrates nuclear immune reprogramming upon chitin perception |
| Description | OsRLCK185 is a functional kinase, we then examined whether OsRLCK185 phosphorylates OsPUB20 using an in vitro kinase assay, OsRLCK185K108A served as a negative control. The results showed that GST-OsRLCK185, but not GST-OsRLCK185K108A, phosphorylates OsPUB20 in vitro. Using LC-MS/MS analysis after an in vitro phosphorylation reaction with GST-OsRLCK185 and His-OsPUB20, we identified threonine 153 (T153), located between U-box domain and ARM repeats of OsPUB20, as the OsRLCK185 phosphorylation site. |
| HostingRepository | iProX |
| AnnounceDate | 2026-01-29 |
| AnnouncementXML | Submission_2026-01-29_00:34:28.749.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Chongyang Zhang |
| SpeciesList | scientific name: Oryza sativa; NCBI TaxID: 4530; |
| ModificationList | phosphorylated residue |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-29 00:34:11 | ID requested | |
| ⏵ 1 | 2026-01-29 00:34:29 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Rice blast resistance, U-box E3 ligase, Phosphorylation |
Contact List
| Yuese Ning | |
|---|---|
| contact affiliation | Institute of Plant protection, Chinese Academy of Agricultural Sciences |
| contact email | ningyuese@caas.cn |
| lab head | |
| Chongyang Zhang | |
| contact affiliation | Institute of Plant protection, Chinese Academy of Agricultural Sciences |
| contact email | zcyimok@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
