PXD073725 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | TMT-based quantitative proteomics analysis of PSMα3-treated THP-1 derived macrophages |
| Description | This study employed tandem mass tag (TMT) quantitative proteomics to investigate the dose-dependent effects of Staphylococcus aureus virulence factor phenol-soluble modulin α3 (PSMα3) on the global protein expression profile of human macrophages. THP-1 monocytic cells were differentiated into macrophages using PMA and then treated with 0 (control), 1, or 3 μM PSMα3 for 24 hours. Cellular proteins were extracted, digested, labeled with TMT reagents, and analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) on an Orbitrap Exploris 480 platform. The dataset aims to identify proteins and pathways significantly altered by PSMα3 exposure, providing insights into the molecular mechanisms underlying macrophage responses to staphylococcal infection and potential targets for therapeutic intervention. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-05 |
| AnnouncementXML | Submission_2026-02-05_01:42:31.272.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bo Ma |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-01-28 10:35:44 | ID requested | |
| 1 | 2026-01-28 23:32:02 | announced | |
| ⏵ 2 | 2026-02-05 01:42:31 | announced | 2026-02-05: Updated project metadata. |
Publication List
| Dataset with its publication pending |
Keyword List
Contact List
| Jianwei Xie |
|---|
| contact affiliation | Academy of Military Medical Sciences, China |
| contact email | xiejw@bmi.ac.cn |
| lab head | |
| Bo Ma |
|---|
| contact affiliation | Academy of Military Medical Sciences |
| contact email | mbcarl@163.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD073725 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD073725
- Label: PRIDE project
- Name: TMT-based quantitative proteomics analysis of PSMα3-treated THP-1 derived macrophages
