PXD073709

PXD073709 is an original dataset announced via ProteomeXchange.

Title	Quantitative and Site-Specific Profiling of Protein O-GlcNAcylation in Platinum-Based Chemotherapeutics
Description	Platinum (Pt)-based chemotherapeutics is emerging as a promising strategy for cancer treatment in clinical trials. Protein O-linked β-N-acetylglucosamine (O-GlcNAc) modification has been reported to occur on several important proteins implicated in regulating cancer resistance to Pt drugs. However, the O-GlcNAc proteomic landscape during this process remains poorly characterized. Herein, we report quantitative profiling of O-GlcNAcylation sites with carboplatin exposure, by using a chemoenzymatic labeling-assisted chemoproteomic approach. The stoichiometric differences of 169 O-GlcNAcylated sites are quantified, many of which occur on essential genome stability regulators. Furthermore, we discover that the cellular O-GlcNAc level is elevated upon carboplatin treatment and suppression of O-GlcNAcylation sensitizes cancer cells to carboplatin-induced cytotoxicity. These results establish a valuable resource for elucidating the function role of O-GlcNAcylation in drug resistance and shed new light on cancer chemotherapy. The chemoproteomic strategy should be generally applicable for monitoring O-GlcNAc dynamic changes in various pharmacological processes.
HostingRepository	PRIDE
AnnounceDate	2026-06-25
AnnouncementXML	Submission_2026-06-24_23:42:49.762.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qingsong Xie
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-28 05:25:54	ID requested
⏵ 1	2026-06-24 23:42:50	announced

10.1021/ACS.JPROTEOME.6C00333;

submitter keyword: O-linked β-N acetylglucosamine (O-GlcNAc),Cancer, LC-MS/MS

Yi Hao
contact affiliation	National Glycoengineering Research Center, Shandong University, Qingdao 266237, Shandong, China.
contact email	yihao@sdu.edu.cn
lab head
Qingsong Xie
contact affiliation	Shandong
contact email	202320520@mail.sdu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073709
     1. Label: PRIDE project
     2. Name: Quantitative and Site-Specific Profiling of Protein O-GlcNAcylation in Platinum-Based Chemotherapeutics