PXD073687

PXD073687 is an original dataset announced via ProteomeXchange.

Title	Domain-selective folding of the tandem β-propeller protein Coronin 7 by the chaperonin CCT/TRiC
Description	The Chaperonin containing tailless complex polypeptide 1 (CCT) or TCP-1 ring complex (TRiC) plays a central role in maintaining cellular homeostasis by supporting protein folding and damping protein aggregation. Besides the abundant cytoskeletal proteins, actin and tubulin, CCT/TRiC is emerging as an obligate chaperone for the β-propeller domain of WD40 proteins. To date, only WD40 proteins consisting of a single β-propeller domain have been described as CCT/TRiC substrates. Using a combination of biotin proximity ligation, co-immunoprecipitation, and knockdown studies, we here identify the tandem β-propeller protein, Coronin 7 (Coro7), as a novel substrate of CCT/TRiC. This raised the question how CCT/TRiC can fold a protein that is too large to fit into its folding chamber, but consists of two domains that require its folding. Surprisingly, co-immunoprecipitation of truncated Coro7 proteins or cleaved full length Coro7 demonstrated that CCT/TRiC only interacts with the first β-propeller domain (PropA) of Coro7. Further experiments showed that CCT/TRiC preferentially binds to PropA, independently of whether this domain is situated at the N- or C-terminus of Coro7. This strongly suggests that CCT/TRiC does not identify β-propeller substrates by their topology, but instead developed specific ways to recognize β-propeller sequences that require folding.
HostingRepository	PRIDE
AnnounceDate	2026-02-20
AnnouncementXML	Submission_2026-02-20_12:53:20.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DeHaven McCrary
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-27 19:29:54	ID requested
⏵ 1	2026-02-20 12:53:20	announced

10.1016/J.JBC.2026.111258;

submitter keyword: CCT/TRiC, Protein folding, chaperone-mediated folding,Coronin 7, BioID

Silvia Jansen
contact affiliation	Washington University in St. Louis Department of Cell Biology and Physiology
contact email	silvia.jansen@wustl.edu
lab head
DeHaven McCrary
contact affiliation	Washington University in St. Louis Department of Cell Biology and Physiology
contact email	d.mccrary@wustl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD073687

PRIDE project URI

• PRIDE
  1. PXD073687
     1. Label: PRIDE project
     2. Name: Domain-selective folding of the tandem β-propeller protein Coronin 7 by the chaperonin CCT/TRiC