PXD073684

PXD073684 is an original dataset announced via ProteomeXchange.

Title	Integrated Proteomics and Machine Learning Reveal AHNAK as a Discriminative Biomarker of Atrial Fibrillation in Heart Failure
Description	Plasma proteins were digested with trypsin and the resulting peptides were analyzed by nanoLC–MS/MS on an EASY-nLC 1200 system coupled to an Orbitrap Exploris 480 mass spectrometer. Peptides were loaded onto a self-packed C18 reversed-phase column (25 cm × 100 μm i.d.) and separated at 500 nL/min using a linear gradient with solvent A (0.1% formic acid, 2% acetonitrile in water) and solvent B (0.1% formic acid, 90% acetonitrile in water): 4–20% B (0–68 min), 20–32% B (68–82 min), 32–80% B (82–86 min), and 80% B wash (86–90 min). The MS was operated with FAIMS using compensation voltages of −70 V and −45 V. Full MS scans were acquired at 60,000 resolution over m/z 400–1,200, and MS/MS scans at 30,000 resolution (first mass m/z 110). The top 15 precursors were selected for HCD (NCE 27%) with 30 s dynamic exclusion; AGC target was 75%, intensity threshold 10,000 ions/s, and maximum injection time 100 ms. TurboTMT was disabled for label-free quantification. Raw data were processed in Proteome Discoverer v2.4.1.15 and searched against the Homo_sapiens_9606_PR_20201214.fasta database (75,777 entries) with a reverse decoy strategy. Trypsin was specified with up to two missed cleavages; minimum peptide length was 6 aa. Carbamidomethyl (C) was set as a fixed modification; oxidation (M), protein N-terminal acetylation, methionine loss, and methionine loss + acetylation were set as variable modifications (≤3 variable modifications per peptide). Precursor and fragment mass tolerances were 10 ppm and 0.02 Da, respectively. FDR was controlled at <1% at the PSM, peptide, and protein levels. For label-free quantification, peptide intensities were normalized by peptide-wise mean centering across samples followed by within-sample median normalization, and protein abundances were summarized as the median of normalized peptide values per protein for downstream analyses.
HostingRepository	PRIDE
AnnounceDate	2026-06-21
AnnouncementXML	Submission_2026-06-21_09:15:11.343.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhenbang Gu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-27 19:07:22	ID requested
⏵ 1	2026-06-21 09:15:11	announced

Dataset with its publication pending

submitter keyword: Atrial fibrillation, Heart failure, AHNAK protein, Biomarkers, Atrial fibrosis

Chen Liu
contact affiliation	the First Affiliated Hospital of Sun Yat-Sen University
contact email	liuch75@mail.sysu.edu.cn
lab head
Zhenbang Gu
contact affiliation	the First Affiliated Hospital of Sun Yat-sen University
contact email	guzhb5@mail2.sysu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD073684

PRIDE project URI

• PRIDE
  1. PXD073684
     1. Label: PRIDE project
     2. Name: Integrated Proteomics and Machine Learning Reveal AHNAK as a Discriminative Biomarker of Atrial Fibrillation in Heart Failure