PXD073655

PXD073655 is an original dataset announced via ProteomeXchange.

Title	The pterocarpan (+)-PTC mimics microtubule-destabilizing agents by modulating cytoskeletal proteins in metastatic castration-resistant prostate cancer: a proteomic perspective
Description	Treatment of metastatic castration-resistant prostate cancer (mCRPC) remains clinically challenging due to tumor heterogeneity and drug resistance. Microtubule-targeting agents are a mainstay of mCRPC therapy, but limitations persist regarding progression-free survival. Pterocarpans have emerged as promising anti-cancer agents, exerting effects through inhibition of bipolar spindle formation, cell cycle arrest in mitosis, and apoptosis induction. In this study, we evaluated the cytotoxic and proteomic effects of the natural product (+)-PTC in PC-3 cells and compared its activity to nocodazole (microtubule depolymerizer) and monastrol (Eg5 inhibitor). Flow cytometry after 24 hours of treatment with 8.0 µM (+)-PTC or 0.25 µM nocodazole revealed a ~50% reduction in cell proliferation relative to controls. Proteomic analyses (LC-MS/MS, GSEA, PANTHER) showed that (+)-PTC shares over 80% of differentially expressed proteins with nocodazole, with 82% overlap among up-regulated and 84% among down-regulated proteins. Top up-regulated proteins (TTLL3, ANAPC7, PIK3CA, ARID4B, COL16A1) are involved in microtubule dynamics and cell cycling; top down-regulated ones (KDM2B, PTOV1, YWHAQ, PSMB6, DPP6) impact cell survival and checkpoints. Differential expression analyses identified key regulators of mitosis and stress response. These findings nominate (+)-PTC as a candidate for future mCRPC therapy.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:25:03.681.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD073655
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Felipe Sousa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-27 11:22:53	ID requested
⏵ 1	2026-06-28 16:25:04	announced

de Brito Vieira Neto J, Moraes de Farias K, Alves Sales SL, Melo VB, Nascimento Mac, ê, do SM, Koscky Paier CR, Bezerra MJ, Moura AA, Carvalho HF, Domingos de Sousa F, Aquino A, de Oliveira Monteiro AC, Martins-de-Souza D, Banwell MG, Miranda Furtado CL, Pessoa C, The pterocarpan (+)-PTC modulates cytoskeletal proteins and induces apoptosis in metastatic castration-resistant prostate cancer: a proteomic perspective. Front Pharmacol, 17():1770249(2026) [pubmed]

10.6019/PXD073655;

10.3389/fphar.2026.1770249;

submitter keyword: prostate cancer, proteomics, cytoskeleton proteins, pterocarpans, microtubule-targeting agents

Felipe Sousa
contact affiliation	University of Fortaleza
contact email	labem@unifor.br
lab head
Felipe Sousa
contact affiliation	University of Fortaleza
contact email	labem@unifor.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073655
     1. Label: PRIDE project
     2. Name: The pterocarpan (+)-PTC mimics microtubule-destabilizing agents by modulating cytoskeletal proteins in metastatic castration-resistant prostate cancer: a proteomic perspective