PXD073591

PXD073591 is an original dataset announced via ProteomeXchange.

Title	Translational control of germinal center response and plasma cell differentiation by RNA helicase Dhx29
Description	The roles of translational control in the immune system are poorly understood. In this study, we performed CRISPR/Cas9-mediated functional screening of RNA helicases in an in vitro system of plasma cell differentiation and identified Dhx29 as a critical regulator of this process. Mice with B cell-specific deletion of Dhx29 exhibited severely impaired germinal center B cell formation, plasma cell differentiation, and antibody production. Mechanistically, Dhx29 promotes translation of Tcf3 and Tle3 via binding to 5’UTRs of those mRNAs. In the absence of Dhx29, B cells exhibit normal proliferation but fail to undergo class switch to IgG1 and differentiation into plasma cells, resulting in impaired antibody production. Ectopic expression of TCF3 and Tle3 largely restores plasma cell differentiation of Dhx29-deficient B cells. Therefore, this study unravels critical roles of Dhx29 in promoting translation of key transcription factors controlling germinal center response and plasma cell differentiation, discovers a previously unrecognized role of Tle3 in plasma cell differentiation, and illustrates the functional importance of translation control in the immune system.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-23_16:10:06.281.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD073591
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hong Peicheng
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-26 13:36:14	ID requested
⏵ 1	2026-08-23 16:10:07	announced

10.6019/PXD073591;

10.1038/s44318-026-00805-0;

Zhao J, He X, Hong P, Lin L, Du Y, Chen P, Zhang L, Leng J, Ma L, Xie J, Lin X, Adilijiang A, Wang J, Hong Y, Xiao Z, Liu WH, Xiao C, RNA helicase DHX29 controls the translation of transcription factors involved in germinal center response and plasma cell differentiation in mice. EMBO J, 45(13):4605-4635(2026) [pubmed]

submitter keyword: Mus musculus, B cell, LC-MS/MS

Changchun, Xiao
contact affiliation	State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Xiamen, Fujian, China.
contact email	cxiao@scripps.edu
lab head
Hong Peicheng
contact affiliation	Shanghai Jiao Tong University
contact email	jingangtietouwa@sjtu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073591
     1. Label: PRIDE project
     2. Name: Translational control of germinal center response and plasma cell differentiation by RNA helicase Dhx29