PXD073562

PXD073562 is an original dataset announced via ProteomeXchange.

Title	Cell-Type–Resolved Proteomics Reveals Distinct Immune and Metabolic Programs between Primary and Recurrent Ascites in High-Grade Serous Ovarian Cancer
Description	This study presents a cell-type–resolved quantitative proteomics analysis of paired primary and recurrent ascites samples from patients with high-grade serous ovarian carcinoma (HGSOC). Ascites samples were collected from three patients (IROC60, IROC65, and IROC126) at diagnosis and upon disease recurrence following platinum–taxane chemotherapy. For each sample, ascites-derived cells were fractionated into unsorted, CD45⁻ (tumor-enriched), and CD45⁺ (immune-enriched) populations using magnetic-activated cell sorting. Using label-free LC–MS/MS proteomics, we quantified proteome-wide changes associated with disease recurrence across distinct cellular compartments. Pathway-level analyses revealed consistent suppression of interferon signaling across tumor and immune fractions, alongside enhanced oxidative phosphorylation and metabolic reprogramming in recurrent tumor cells. Immune-enriched fractions exhibited signatures of immunosuppression, including reduced antigen presentation and enrichment of regulatory and monocyte-related pathways. Together, this dataset provides a comprehensive proteomic resource for investigating tumor–immune interactions and recurrence-associated molecular remodeling within the ovarian cancer ascites microenvironment.
HostingRepository	PRIDE
AnnounceDate	2026-08-18
AnnouncementXML	Submission_2026-08-18_06:33:31.412.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Goodlett
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-26 05:49:44	ID requested
⏵ 1	2026-08-18 06:33:31	announced

Dataset with its publication pending

submitter keyword: None

David Goodlett
contact affiliation	University of Victoria
contact email	goodlett@uvic.ca
lab head
David Goodlett
contact affiliation	University of Victoria
contact email	goodlettlabuvic@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073562
     1. Label: PRIDE project
     2. Name: Cell-Type–Resolved Proteomics Reveals Distinct Immune and Metabolic Programs between Primary and Recurrent Ascites in High-Grade Serous Ovarian Cancer