PXD073552

PXD073552 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Lauroylation-assisted workflow for N-terminomics with three biological replicates
Description	Lauroylation-assisted N-terminomics for profiling protein N-termini, including ORF and non-ORF protein N-termini, using three biological replicates of etoposide-treated cholangiocarcinoma cells and exploiting negative selection.
HostingRepository	jPOST
AnnounceDate	2026-05-01
AnnouncementXML	Submission_2026-04-30_18:50:23.169.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD073552
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hiroki Okanishi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; Acetyl; unknown modification; N6,N6-dimethyl-L-lysine; alpha-amino dimethylated residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-26 01:43:36	ID requested
⏵ 1	2026-04-30 18:50:24	announced

Publication List

Okanishi H, Uno I, Nishimura SI, Kanai Y, Masui R, A Sensitive, Specific, and Cost-Effective Lauroylation-Assisted Workflow for Profiling Peptide-Level Protease Specificity Using Proteomic Identification of Cleavage Sites (PICS), with Applicability to Protein-Level N-Terminomics. J Proteome Res, 25(4):1878-1891(2026) [pubmed]

Okanishi H, Uno I, Nishimura SI, Kanai Y, Masui R, A Sensitive, Specific, and Cost-Effective Lauroylation-Assisted Workflow for Profiling Peptide-Level Protease Specificity Using Proteomic Identification of Cleavage Sites (PICS), with Applicability to Protein-Level N-Terminomics. J Proteome Res, 25(4):1878-1891(2026) [pubmed]

Keyword List

submitter keyword: Lauroylation, Protease, Protein N-termini, Cleavage site

submitter keyword: Lauroylation, Protease, Protein N-termini, Cleavage site

Contact List

Ryoji Masui, Yoshikatsu Kanai
lab head
Hiroki Okanishi
contact affiliation	Department of Bio-system Pharmacology, Graduate School of Medicine, Osaka University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004352/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004352/