PXD073534

PXD073534 is an original dataset announced via ProteomeXchange.

Title	Heat stress triggers nuclear invagination and spatial compartmentalization of protein metabolism
Description	Heat stress is a common challenge for cells, causing multiple types of cellular damage while triggering complex stress responses, including the classical heat shock response. However, the subcellular coordination of these stress responses remains poorly understood. In this study, we identify reversible nuclear morphological changes under heat stress, characterized by kidney-shaped invaginations. These nuclear invaginations are associated with intermediate filament collapse and the regional clustering of organelles. Through immunofluorescence imaging and proteomic analysis, we further reveal that nuclear invagination region function as specialized compartments where newly synthesized proteins are concentrated and protein degradation demand is heightened. Moreover, this compartmentalization is not only essential for cellular adaptation and recovery from heat stress but also correlates with the differential heat tolerance across cell lines. Our findings highlight a previously unappreciated mechanism by which cells spatially reorganize protein metabolism to optimize stress responses, providing new insights into heat stress adaptation.
HostingRepository	iProX
AnnounceDate	2026-01-23
AnnouncementXML	Submission_2026-01-25_22:59:42.135.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhihao Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-25 22:59:25	ID requested
⏵ 1	2026-01-25 22:59:42	announced

Dataset with its publication pending

submitter keyword: heat stress, nuclear invagination, protein metabolism

Guanzheng Luo
contact affiliation	Sun Yat-sen University
contact email	luogzh5@mail.sysu.edu.cn
lab head
Zhihao Zhang
contact affiliation	Sun Yat-sen University
contact email	zhangzhh63@mail2.sysu.edu.cn
dataset submitter

iProX dataset URI