PXD073462

PXD073462 is an original dataset announced via ProteomeXchange.

Title	Outer Membrane Vesicles Secreted by Bacteroides fragilis Inhibit CFTR Chloride Secretion by Human Colon Organoids
Description	The goals of this study were to develop a model to study host pathogen interactions in primary human colon organoids and to test the hypothesis that Bacteroides fragilis toxin (BFT-2) secreted in outer membrane vesicles (OMVs) modulates mucosal immunity and CFTR Cl- secretion. Since Bacteroides species often resides in mucus, OMVs are likely to represent a mechanism of communication between Bacteroides and the host. Two strains of Bacteroides were studied, Enterotoxigenic Bacteroides fragilis (ETBF), which produces BFT-2, and the non-toxigenic Bacteroides fragilis strain NCTC 9343 (NTBF) that does not produce BFT-2. We also utilized two additional strains of Bacteroides fragilis: one in which bft-2 was knocked out (ETBF Δbft), and one that was engineered to contain bft-2 (NTBF+bft). We report that most Bacteroides fragilis OMVs reduced CFTR Cl- secretion but had no effect on tight junction or cell adhesion proteins, transepithelial resistance (TER) or cytokine secretion by primary human colon organoids. We conclude that OMVs secreted by Bacteroides can be an important mechanism of host pathogen interactions in the colon by reducing CFTR Cl- secretion.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_09:39:27.578.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Byoung-Kyu Cho
SpeciesList	scientific name: Bacteroides fragilis; NCBI TaxID: NEWT:817;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-23 09:28:53	ID requested
⏵ 1	2026-05-21 09:39:28	announced

Dataset with its publication pending

submitter keyword: transepithelial resistance, CFTR,Bacteroides fragilis, colon organoids, outer membrane vesicles

Young Ah Goo
contact affiliation	Mass Spectrometry Technology Access Center, McDonnell Genome Institute, Washington University School of Medicine
contact email	ygoo@wustl.edu
lab head
Byoung-Kyu Cho
contact affiliation	Washington University in St. Louis
contact email	byoung-kyu@wustl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073462

PRIDE project URI

• PRIDE
  1. PXD073462
     1. Label: PRIDE project
     2. Name: Outer Membrane Vesicles Secreted by Bacteroides fragilis Inhibit CFTR Chloride Secretion by Human Colon Organoids