PXD073444

PXD073444 is an original dataset announced via ProteomeXchange.

Title	Seasonal trade-offs shape metabolomic and proteomic responses to Varroa destructor parasitisation in honey bees
Description	The ectoparasitic mite Varroa destructor is a major driver of honey bee mortality, yet its impact depends on the specific phenotypes of summer and winter worker bees. Here, we apply an LC-MS/MS-based proteomics to dissect how Varroa parasitisation affects the molecular physiology of short-lived summer and long-lived winter worker bees. Newly generated proteomic data from summer bees were integrated with a previously published winter bees dataset (PXD030369), enabling direct seasonal comparison under identical analytical pipelines. Heads and abdomens from two experimental groups (varroa parasitised labelled as “Varroa”, healthy control labelled as “Unpara”) and two seasonal phenotypes (summer and winter) were compared. Despite strong seasonal divergence, a conserved Varroa-associated proteomic changes emerged across seasons, characterised by enhanced cellular logistics and membrane remodelling coupled with compromised metabolic robustness and nutritional output. Together, our results demonstrate that V. destructor does not elicit a uniform stress response but exploits seasonally distinct physiological states of honey bees, generating divergent molecular stress patterns with direct implications for worker survival and colony resilience.
HostingRepository	PRIDE
AnnounceDate	2026-03-12
AnnouncementXML	Submission_2026-03-11_17:18:12.277.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD073444
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jacek Marciniak
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: NEWT:7460;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-23 03:30:11	ID requested
⏵ 1	2026-03-11 17:18:12	announced

10.6019/PXD073444;

10.1016/J.IBMB.2026.104535;

submitter keyword: None

Pavel Dobes
contact affiliation	Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
contact email	pavel.dobes@mail.muni.cz
lab head
Jacek Marciniak
contact affiliation	Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic
contact email	marciniak@sci.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD073444

PRIDE project URI

• PRIDE
  1. PXD073444
     1. Label: PRIDE project
     2. Name: Seasonal trade-offs shape metabolomic and proteomic responses to Varroa destructor parasitisation in honey bees