⮝ Full datasets listing
PXD073439
PXD073439 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | In-depth Phosphoproteome Analysis from Microscale Samples with Rapid In-Situ One-Tip IMAC Strategy |
| Description | In this study, we developed a simple and rapid in-situ one-tip IMAC phosphoproteomics workflow integrated with data dependent acquisition without exclusion mass spectrometry for microscale phosphoproteomic analysis. The phosphopeptide enrichment material, made from hydrophilic cotton fibers with immobilized titanium ions, is easy to pack into a pipette tip. It enables fast, efficient enrichment through aspiration and dispensing, minimizing sample loss and enhancing sensitivity. Ti-Cotton tip successfully enriched phosphopeptides from protein standard digests and exhibited a high selectivity (BSA/standard phosphopeptide = 2000:1), and enabled 10,332 phosphosites assigned to 1,992 phosphoproteins from 20,000 HeLa cells. Furthermore, large scale of 36,521 phosphosites assigned to 4,452 phosphoproteins with 8,952 new sites could be identified by using 30 μg HeLa cell lysates as starting material via combination with high-pH RP fractionation. Taken together, our strategy is promising to serve as a widely applicable and robust platform for achieving large-scale and highly sensitive phosphopeptide enrichment and expanding our knowledge of phosphoproteomics in complex microscale biological systems. |
| HostingRepository | iProX |
| AnnounceDate | 2026-01-23 |
| AnnouncementXML | Submission_2026-01-23_00:56:39.084.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ci Wu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-23 00:56:18 | ID requested | |
| ⏵ 1 | 2026-01-23 00:56:39 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Phosphoproteomics |
| Microscale analysis |
| Immobilized metal affinity chromatography |
| Sensitivity |
| Phosphopeptide enrichmen |
Contact List
| Ci Wu | |
|---|---|
| contact affiliation | Liaoning Normal University |
| contact email | wuci1111@126.com |
| lab head | |
| Ci Wu | |
| contact affiliation | Liaoning Normal University |
| contact email | wuci1111@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
