PXD073432

PXD073432 is an original dataset announced via ProteomeXchange.

Title	Proximity labeling of proteome and metabolome at organelle membranes with split-PLD reveals hydrogen sulfide-inhibited cuproptosis
Description	Crosslinking mass spectrometry has been widely applied to probe protein–protein interactions. However, proximity labeling at organelle membranes, particularly beyond proteins, is still poorly characterized. Here, we develop a splitted phospholipase D (split-PLD)–based proximity labeling strategy targeted to mitochondrial and endoplasmic reticulum (ER) membranes, enabling the identification of substantially more membrane-associated proteins than existing methods such as TurboID and APEX2, and revealing proximal proteome atlas with different distances from organelle membranes, including functional assemblies. Importantly, integration of the split-PLD-driven labeling mechanism with click chemistry allows interrogation of proximal reactions between metabolites and lipids at mitochondrial and ER membranes without organelle isolation. Using this platform, we identified a gas molecule hydrogen sulfide (H2S) at mitochondrial membrane interfaces and demonstrate that H2S suppresses cuproptosis in the presence of copper ionophore elesclomol. Collectively, we establish a versatile platform for interrogating molecular activities at organellar membranes, further conceptualizing “contactomics”.
HostingRepository	iProX
AnnounceDate	2026-01-22
AnnouncementXML	Submission_2026-01-23_00:44:35.020.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianlong Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-23 00:44:16	ID requested
⏵ 1	2026-01-23 00:44:35	announced

Dataset with its publication pending

submitter keyword: Organelle Membrane

Proximity labeling

Phospholipase D

Proteomics

Metabolomics

Yaoyang Zhang
contact affiliation	Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
contact email	zyy@sioc.ac.cn
lab head
Jianlong Li
contact affiliation	Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
contact email	lijianlong@sioc.ac.cn
dataset submitter

iProX dataset URI