PXD073421

PXD073421 is an original dataset announced via ProteomeXchange.

Title	The role of ANKRD9 in mouse jejunal enteroids.
Description	The absorption of dietary fats represents one of the most energy-intensive processes involved in nutrient uptake. Processes such as fatty acid activation, triglyceride synthesis, and the trafficking of chylomicrons through the secretory pathway require ATP. The mechanism by which enterocytes manage the increased ATP demand following fat absorption remains unclear. Ankyrin Repeat Domain 9 (ANKRD9) has been identified as a fat responsive protein. Our research revealed that the inactivation of Ankrd9 results in a significant decrease in ATP levels within enterocytes, without affecting mitochondrial function or glycolysis, and leads to increased lipid accumulation, thereby reducing body fat mass. These findings suggest that ANKRD9 plays a crucial role in the regulation of ATP and fat processing. To further elucidate the function of ANKRD9 in enterocytes, we conducted proteomic analyses of jejunal differentiated enteroids derived from wild-type (Ankrd9+/+) and Ankrd9 knockout (Ankrd9-/-) mice.
HostingRepository	PRIDE
AnnounceDate	2026-03-17
AnnouncementXML	Submission_2026-03-17_02:54:56.404.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chanhyun na
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-22 15:50:45	ID requested
1	2026-03-12 13:32:01	announced
⏵ 2	2026-03-17 02:54:56	announced	2026-03-17: Updated project metadata.

Dataset with its publication pending

submitter keyword: mouse, TMT, Ankrd9 knockout, enteroid

Chan-Hyun Na
contact affiliation	Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
contact email	chanhyun@jhmi.edu
lab head
chanhyun na
contact affiliation	Johns Hopkins University
contact email	chanhyun.na@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073421
     1. Label: PRIDE project
     2. Name: The role of ANKRD9 in mouse jejunal enteroids.