PXD073419

PXD073419 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	ZNFX1 activity-based probe crosslinking mass spectrometry
Description	The aim of this experiment was to identify the catalytic cysteine in the antiviral E3 ligase ZNFX1 using activity-based probe (ABP)–enabled cross-linking mass spectrometry (XL-MS). A biotinylated E2~ubiquitin activity-based probe containing a cysteine-electrophile trap was used to covalently capture ubiquitin a transfer intermediate between UBE2D2~Ub and full-length human ZNFX1. Cross-linked peptide mapping was performed to identify probe-labelled residues and putative catalytic site.
HostingRepository	PRIDE
AnnounceDate	2026-05-22
AnnouncementXML	Submission_2026-05-22_10:50:28.405.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Satpal Virdee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-22 11:52:08	ID requested
⏵ 1	2026-05-22 10:50:29	announced

Publication List

Squair DR, Rivers E, Sowar H, Balci A, Harmo R, Wright DJ, Beniwal G, Soetens M, Mathur S, Tollervey A, Wood NT, Pao KC, Stanton C, Fletcher AJ, Virdee S, ZNFX1 uses two-component ubiquitin circuitry to quarantine viral RNA. Mol Cell, 86(6):1164-1181.e12(2026) [pubmed]
10.1016/j.molcel.2026.02.015;

Squair DR, Rivers E, Sowar H, Balci A, Harmo R, Wright DJ, Beniwal G, Soetens M, Mathur S, Tollervey A, Wood NT, Pao KC, Stanton C, Fletcher AJ, Virdee S, ZNFX1 uses two-component ubiquitin circuitry to quarantine viral RNA. Mol Cell, 86(6):1164-1181.e12(2026) [pubmed]

10.1016/j.molcel.2026.02.015;

Keyword List

submitter keyword: None

submitter keyword: None

Contact List

Satpal Virdee
contact affiliation	MRC Protein Phosphorylation and Ubiquitylation Unit, University of Dundee, UK
contact email	s.s.virdee@dundee.ac.uk
lab head
Satpal Virdee
contact affiliation	University of Dundee
contact email	s.s.virdee@dundee.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073419
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD073419

PRIDE project URI

Repository Record List

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