PXD073416

PXD073416 is an original dataset announced via ProteomeXchange.

Title	Intact mass spectrometry analysis of covalent binding between purified recombinant human FGFR2 V564F protein and small-molecule compound LC-F2-1 incubated at a molar ratio of 1:2 or 1:5 at room temperature via Agilent 6230 time-of-flight LC/MS system for the characterization of their covalent binding products in protein-drug interaction studies
Description	This dataset corresponds to an intact mass spectrometry study focusing on the covalent binding interaction between purified FGFR2 V564F protein and compound LC-F2-1. The purified FGFR2 V564F was adjusted to 28 μM, then incubated with LC-F2-1 at molar ratios of 1:2 or 1:5 for 4 hours at room temperature. Preliminary LC-MS analysis confirmed that all FGFR2 V564F molecules formed covalent adducts with LC-F2-1 after incubation. Subsequently, intact mass analysis was conducted using an Agilent 6230 time-of-flight LC/MS system (equipped with Dual AJS ESI), and protein samples were desalted via an Agilent 1290 Infinity II instrument prior to mass spectrometry detection. BioConfirm software was applied to deconvolute the protein spectra, aiming to characterize the covalent binding product of FGFR2 V564F and LC-F2-1.
HostingRepository	PRIDE
AnnounceDate	2026-09-14
AnnouncementXML	Submission_2026-09-13_18:10:05.773.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiansheng Cao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	6220 Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-22 10:39:21	ID requested
⏵ 1	2026-09-13 18:10:06	announced

10.1038/s41467-026-76339-0;

submitter keyword: Covalent binding, LC-MS, LC-F2-1,FGFR2 V564F

Lingfeng Chen
contact affiliation	School of Pharmacy, Hangzhou Medical College, Hangzhou 310014, Zhejiang, 8 China
contact email	lfchen@hmc.edu.cn
lab head
Xiansheng Cao
contact affiliation	Hangzhou Medical College
contact email	18822629637@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073416
     1. Label: PRIDE project
     2. Name: Intact mass spectrometry analysis of covalent binding between purified recombinant human FGFR2 V564F protein and small-molecule compound LC-F2-1 incubated at a molar ratio of 1:2 or 1:5 at room temperature via Agilent 6230 time-of-flight LC/MS system for the characterization of their covalent binding products in protein-drug interaction studies