⮝ Full datasets listing
PXD073382
PXD073382 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Optogenetic Control of OGT Membrane Localization Reveals Spatiotemporal Regulation of O-GlcNAcylation and Its Crosstalk with Phosphorylation in Cellular Signaling |
| Description | This project investigates the spatiotemporal dynamics of protein O-GlcNAcylation mediated by optogenetically controlled localization of O-GlcNAc transferase (OGT) to the plasma membrane. Cells expressing the OGT optogenetic system were subjected to varied light intensities (0, 0.2, and 1 mW/cm² for 10 s) or exposure durations (1 mW/cm² for 0, 1, and 10 s). Cell membranes were biochemically fractionated, enzymatically labeled with azido-containing N-acetylgalactosamine (GalNAz), and further conjugated with alkynyl biotin via the click chemistry. Biotinylated proteins were isolated with streptavidin-coated beads and subjected to on-beads trypsin digestion. The resulting peptides were then isotopically derivatized by dimethyl labeling of amino groups by reductive amination, combined and subjected to relative quantification via mass spectrometry. The study reveals how subcellular targeting of OGT rewires signaling networks and provides mechanistic insights into the compartmentalized regulation of O-GlcNAcylation and its crosstalk with other post-translational modifications. |
| HostingRepository | iProX |
| AnnounceDate | 2026-01-21 |
| AnnouncementXML | Submission_2026-01-21_19:16:58.998.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhiya Fan |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-21 19:16:37 | ID requested | |
| ⏵ 1 | 2026-01-21 19:16:59 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: O-GlcNAcylation, OGT, optogenetics, glycoproteomics, membrane localization |
Contact List
| Weijie Qin | |
|---|---|
| contact affiliation | State Key Laboratory of Medical Proteomics, National Center for Protein Sciences (Beijing), Academy of Military Medical Sciences |
| contact email | aunp_dna@126.com |
| lab head | |
| Zhiya Fan | |
| contact affiliation | State Key Laboratory of Medical Proteomics, National Center for Protein Sciences (Beijing), Academy of Military Medical Sciences |
| contact email | zyfan7@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
