⮝ Full datasets listing
PXD073350
PXD073350 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Redefining the role of the Plasmodium heme detoxification protein: From hemozoin to mitochondrial protein synthesis |
| Description | Malaria blood-stage parasites digest ~80% of host cell hemoglobin within a degradative vacuole, releasing heme that is detoxified by sequestration into hemozoin crystals. Although essential for survival and a validated drug target, the mechanisms of heme biomineralization remain unclear. We studied the parasite’s Heme Detoxification Protein (HDP), previously proposed to mediate hemozoin formation, using genetic, microscopic, bioenergetic, and proteomic approaches. Endogenous tagging revealed that HDP localizes to the mitochondrion, not the vacuole. Conditional HDP inactivation had no effect on heme biomineralization, but caused mitochondrial depolarization, hypersensitivity to proguanil, and developmental arrest, which was rescued by bypassing respiratory-chain-dependent pyrimidine biosynthesis. HDP knockout abolished mitochondrial electron flow due to loss of complexes III and IV, consistent with impaired mitochondrial protein synthesis. Integration of structural modelling with quantitative proteomics placed HDP within the mitoribosomal large subunit. Here, we show that HDP is essential for mitochondrial function and does not contribute to hemozoin formation. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-06-29 |
| AnnouncementXML | Submission_2026-06-29_07:26:28.825.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tanja Ziesmann |
| SpeciesList | scientific name: Plasmodium falciparum; NCBI TaxID: 5833; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-21 07:59:03 | ID requested | |
| ⏵ 1 | 2026-06-29 07:26:29 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: malaria, Plasmodium, HDP, heme, hemozoin, mitochondrion, ribosome, electron transport chain |
Contact List
| Joachim M. Matz | |
|---|---|
| lab head | |
| Tanja Ziesmann | |
| contact affiliation | Insitute of Immunology, University Medical Center Mainz |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004216/ |
