PXD073301

PXD073301 is an original dataset announced via ProteomeXchange.

Title	New Frontiers in the Biological Cardiac Rhythm Generator: Preserving Atrioventricular Node Architecture in Decellularized Scaffolds
Description	Cardiac implantable electronic devices are the current standard for managing arrhythmias, yet their use remains limited bydevice-related mechanical failures , infection risk, and suboptimal long-term biocompatibility. Developing a biological alternative capable of restoring intrinsic pacemaking function therefore represents an important clinical and technological challenge. In this study, we report the development of a bioengineered cardiac scaffold derived from porcine atrioventricular nodes. A Tergitol-based decellularization protocol was optimized to achieve complete removal of cellular and nuclear components while preserving the three-dimensional architecture and key extracellular matrix constituents. Comprehensive morphological, ultrastructural, proteomic and mechanical analyses confirmed ECM integrity and maintenance of structural organization, including collagen preservation with mild fiber realignment. These results demonstrate that the proposed decellularization strategy yields an ECM scaffold that retains the native features essential for supporting pacemaker tissue architecture. This work lays the groundwork for the creation of next-generation, tissue-derived cardiac scaffolds, with ongoing efforts directed toward ECM-derived hydrogel fabrication, cytocompatibility evaluation, and future integration with cardiomyocytes for biologically inspired pacemaker applications.
HostingRepository	PRIDE
AnnounceDate	2026-02-20
AnnouncementXML	Submission_2026-02-20_08:08:58.541.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna Maria Tolomeo
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-20 15:57:41	ID requested
⏵ 1	2026-02-20 08:08:59	announced

10.3389/FBIOE.2026.1766378;

submitter keyword: pacemaker,ECM,heart

Anna Maria Tolomeo
contact affiliation	TIGEM
contact email	annamaria.tolomeo@unipd.it
lab head
Anna Maria Tolomeo
contact affiliation	Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, Padua, Italy
contact email	annamaria.tolomeo@unipd.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD073301

PRIDE project URI

• PRIDE
  1. PXD073301
     1. Label: PRIDE project
     2. Name: New Frontiers in the Biological Cardiac Rhythm Generator: Preserving Atrioventricular Node Architecture in Decellularized Scaffolds