PXD073243

PXD073243 is an original dataset announced via ProteomeXchange.

Title	A new family of ribosome hibernation factors in ArchaeaA new family of ribosome hibernation factors in Archaea
Description	Ribosome hibernation preserves translation machinery during stress, yet its mechanisms in Archaea remain poorly defined. Here, we identify HibA, a previously unrecognized family of hibernation factors widespread in Archaea. HibA consists of a bacterial-like HPF/RaiA domain fused to a Cystathionine Beta Synthase module. High-resolution cryo-EM structures of HibA:ribosome complexes from Pyrococcus abyssi identify three distinct conformations overlapping A, P and E site tRNA positions. Thus, HibA can inactivate ribosomes in multiple conformational states, providing a structurally robust mechanism for ribosome preservation. Idle ribosomes also frequently accumulate SBDS, suggesting that stressed archaeal cells may engage parallel hibernation routes in which SBDS can complement HibA. Deletion of hibA in Thermococcus barophilus delays recovery from stationary phase and reduces 70S ribosome pools, establishing its role in ribosome preservation. Taxonomic profiling shows that many archaeal lineages encode multiple ribosome-associated protection factors, underscoring the multi-layered nature of archaeal hibernation systems. In addition, a comprehensive phylogenetic analysis highlights the evolutionary relationships between prevalent ribosome hibernation factors across Bacteria and Archaea.
HostingRepository	PRIDE
AnnounceDate	2026-03-24
AnnouncementXML	Submission_2026-03-24_03:38:29.432.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magalie Duchateau
SpeciesList	scientific name: Pyrococcus abyssi GE5; NCBI TaxID: NEWT:272844;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-19 07:59:19	ID requested
⏵ 1	2026-03-24 03:38:29	announced

Dataset with its publication pending

submitter keyword: Archaea,ribosome, hibernation

Emmanuelle Schmitt
contact affiliation	Laboratoire de Biologie Structurale de la Cellule, BIOC, CNRS, Ecole polytechnique, Institut Polytechnique de Paris, 91120, Palaiseau, France
contact email	emmanuelle.schmitt@polytechnique.edu
lab head
Magalie Duchateau
contact affiliation	Institut Pasteur
contact email	magalie.duchateau@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD073243
     1. Label: PRIDE project
     2. Name: A new family of ribosome hibernation factors in ArchaeaA new family of ribosome hibernation factors in Archaea