⮝ Full datasets listing
PXD073027
PXD073027 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CRISPR screening identifies GNPTAB as a noncanonical STING activator driving cellular senescence |
| Description | Cellular senescence is characterized by profound lysosomal alterations, yet whether lysosome-associated factors actively drive aging remains unclear. Through a focused CRISPR/Cas9 screen in human mesenchymal progenitor cells (hMPCs), we identify GNPTAB, an enzyme for lysosomal hydrolase targeting, as a potent regulator of senescence. Genetic ablation of GNPTAB attenuated senescence, while its overexpression accelerated aging. Notably, this pro-senescent function was independent of GNPTAB’s canonical enzymatic role. Mechanistically, GNPTAB binds to the innate immune adaptor STING via a specific interface (E1119), triggering downstream TBK1 activation and inflammatory gene expression. A STING-binding-deficient GNPTAB mutant (E1119A) retained enzymatic function but failed to induce senescence, and STING depletion abolished GNPTAB-driven senescence. Our work defines a non-canonical, lysosome-to-immune signaling pathway wherein GNPTAB acts as a direct STING activator, nominating this interface as a candidate target for mitigating age-related cellular dysfunction. |
| HostingRepository | iProX |
| AnnounceDate | 2026-01-11 |
| AnnouncementXML | Submission_2026-01-13_17:53:31.649.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yizhou Gao |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-13 17:53:18 | ID requested | |
| ⏵ 1 | 2026-01-13 17:53:32 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: CRISPR screening, aging, cellular senescence, lysosomal biology |
Contact List
| Jing Qu | |
|---|---|
| contact affiliation | Institute of Zoology, Chinese Academy of Sciences |
| contact email | qujing@ioz.ac.cn |
| lab head | |
| Yizhou Gao | |
| contact affiliation | Institute of Zoology, Chinese Academy of Sciences |
| contact email | 2412676398@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
