PXD073018

PXD073018 is an original dataset announced via ProteomeXchange.

Title	Treatment of human oocytes with extracellular vesicles from follicular fluid during rescue in vitro maturation enhances maturation rates and modulates oocyte proteome and ultrastructure
Description	This project aimed to elucidate whether human follicular fluid–derived extracellular vesicles (ffEVs) enhance oocyte maturation during rescue in vitro maturation (rIVM). We characterised the protein cargo of ffEVs isolated from follicles containing oocytes at different maturation stages (GV, GVBD, and MII), identifying distinct proteomic signatures associated with follicular competence and oocyte maturity. Subsequently, single-cell proteomic profiling of IVM-matured oocytes supplemented with ffEVs demonstrated that ffEV treatment induces significant changes in oocyte protein abundance, including factors linked to oocyte maturation, endoplasmic reticulum function, steroid biosynthesis, and cytoskeletal organisation. These proteomic alterations were accompanied by changes in oocyte ultrastructure and organelle architecture.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:52:28.705.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mara Saenz-de-Juano
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-13 11:40:08	ID requested
⏵ 1	2026-04-05 16:52:29	announced

10.1093/hropen/hoag021;

Makieva S, Saenz-de-Juano MD, Almi, ñ, ana C, Bauersachs S, Bernal-Ulloa S, Xie M, Velasco AG, Cervantes N, Sachs M, Ulbrich SE, Leeners B, maturation enhances maturation rates and modulates oocyte proteome and ultrastructure. Hum Reprod Open, 2026(2):hoag021(2026) [pubmed]

submitter keyword: follicular fluid, extracellular vesicles,Human oocyte, in vitro maturation, single-cell proteomic analysis

Prof. Dr. Susanne Ulbrich
contact affiliation	ETH Zürich, Department of Environmental Systems Science
contact email	susanne.ulbrich@usys.ethz.ch
lab head
Mara Saenz-de-Juano
contact affiliation	ETH Zurich
contact email	mara.saenz@usys.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD073018

PRIDE project URI

• PRIDE
  1. PXD073018
     1. Label: PRIDE project
     2. Name: Treatment of human oocytes with extracellular vesicles from follicular fluid during rescue in vitro maturation enhances maturation rates and modulates oocyte proteome and ultrastructure