PXD072976

PXD072976 is an original dataset announced via ProteomeXchange.

Title	Anti-tumor bioactive component identification
Description	The anti-tumor effect of different F. prausnitzii strains were evaluated using classic azoxymethane plus dextran sulfate sodium (AOM/DSS) and Apcmin/+ colorectal tumorigenesis mouse models, which allowed us to identify the bioactive component in the potent F. prausnitzi. With the classic tumor coculture model, we found that F. prausnitzii extracts induced strongest anti-tumor effects comparing to F. prausnitzii lysate and the culture supernatant. We next confirmed that the bioactive component was protein in nature as demonstrated by its loss of activity upon protease digestion and further verified that it was enriched in the fraction with molecular weight greater than 30 kDa. Interestingly, different treatment approaches revealed that pre-treating MC38 tumor cells with F. prausnitzii extracts elicited the strongest anti-tumor response, compared to pre-treating splenocytes or direct coculture. Extracted protein was analyzed by mass spectrum (MS) and F. prausnitzii-originated PRPS was further identified.
HostingRepository	iProX
AnnounceDate	2026-01-13
AnnouncementXML	Submission_2026-01-12_17:36:47.766.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuai Zhang
SpeciesList	scientific name: Faecalibacterium prausnitzii; NCBI TaxID: 853;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-12 17:36:23	ID requested
⏵ 1	2026-01-12 17:36:48	announced

Dataset with its publication pending

submitter keyword: bioactive component

Mingming Zhang
contact affiliation	Shanghai Jiao Tong University
contact email	doczmm@sjtu.edu.cn
lab head
Shuai Zhang
contact affiliation	Huazhong Agricultural University
contact email	shuaizhang@mail.hzau.edu.cn
dataset submitter

iProX dataset URI