PXD072961

PXD072961 is an original dataset announced via ProteomeXchange.

Title	Isolation and proteomic analysis of intracellular vesicles from the potato late blight pathogen Phytophthora infestans
Description	Phytophthora infestans is a major pathogen of potato and tomato, a key determinant to the successful outcome of disease laying with the effector proteins secreted by the pathogen during infection; these collectively act to facilitate evasion of plant immunity, physical breaching of the plant tissue, and nutrient acquisition by the pathogen. These effector proteins can act within the host apoplast/extracellular space (apoplastic effectors) or are translocated into, and act within, the host cell (cytoplasmic effectors). There has been much interest in the fate and function of these proteins once secreted into the host plant, with the intracellular trafficking of these proteins enroute to secretion neglected. We combined differential centrifugation with cushioned bottom loaded gradient ultracentrifugation to isolate intracellular vesicles from in vitro grown P. infestans based on buoyancy. A transgenic P. infestans line expressing two fluorescently tagged effector proteins, PITG_04314-mCherry and PITG_01029-mCitrine, representing potential intracellular vesicle cargo enabled identification of the gradient fractions to which these proteins migrated. Mass spectrometry analysis of gradient fraction containing the majority of the two tagged effector proteins showed significant association with proteins associated with membranes and membrane trafficking. The method also identified the presence of known P. infestans extracellular vesicle markers (three tetraspanins and two MARVEL domain proteins), suggesting the capture of vesicles enroute to extracellular secretion.
HostingRepository	PRIDE
AnnounceDate	2026-02-13
AnnouncementXML	Submission_2026-02-13_05:37:02.159.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jasmine Pham
SpeciesList	scientific name: Phytophthora infestans T30-4; NCBI TaxID: NEWT:403677;
ModificationList	dihydroxylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-12 09:13:20	ID requested
⏵ 1	2026-02-13 05:37:02	announced

10.1038/S41598-026-37161-2;

submitter keyword: None

Paul Birch
contact affiliation	University of Dundee, School of Life Sciences
contact email	p.birch@dundee.ac.uk
lab head
Jasmine Pham
contact affiliation	University of Dundee
contact email	j.pham@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD072961

PRIDE project URI

• PRIDE
  1. PXD072961
     1. Label: PRIDE project
     2. Name: Isolation and proteomic analysis of intracellular vesicles from the potato late blight pathogen Phytophthora infestans