PXD072946

PXD072946 is an original dataset announced via ProteomeXchange.

Title	DUSP12 promotes cell cycle progression and protects cells from cells from ZNF622 mediated apoptosis
Description	Protein phosphatases are critical for regulating cell signaling, cell cycle, and cell fate decisions, and their dysregulation leads to an array of human diseases like cancer. The dual specificity phosphatases (DUSPs) have emerged as important factors driving tumorigenesis and cancer therapy resistance. DUSP12 is a poorly characterized atypical DUSP widely conserved throughout evolution. Although no direct substrate has been firmly established, DUSP12 has been implicated in protecting cells from stress, regulating ribosomal biogenesis, and modulating cellular DNA content. In this study, we used affinity- and proximity-based biochemical purification approaches coupled to mass spectrometry to identify the zinc finger protein ZNF622 as a novel DUSP12 interactor, which was validated by in cell and in vitro IP assays. Interestingly, ZNF622 binds to the unique zinc-binding domain of DUSP12, which previous reports indicated was important for many of DUSP12’s functions within the cell. Prior studies had implicated ZNF622 as a modulator of apoptosis, but it remained unclear if and how ZNF622 participated in the cell cycle and, more so, how it promoted cell death. Using mass spectrometry analyses, we found that overexpression of DUSP12 promoted de-phosphorylation of ZNF622 at Ser 143 . Overexpression of ZNF622, but not Ser 143 phosphomimetic and phosphorylation-deficient mutants, led to an increase in pre-metaphase mitotic defects while knockdown of DUSP12 also showed mitotic defects in metaphase. Furthermore, knockdown of DUSP12 promoted, while knockdown of ZNF622 suppressed, stress-induced apoptosis. Our results support a model where DUSP12 protects cells from ZNF622 mediated stress-induced apoptosis.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_18:41:25.355.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mehdi Bouhaddou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	phosphorylated residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-12 02:41:43	ID requested
⏵ 1	2026-04-05 18:41:26	announced

10.1038/s41419-026-08618-z;

Abdusamad M, Guo X, Ramirez I, Velasquez EF, Cohn W, Gholkar AA, Ashley IA, Delgado Y, Bouhaddou M, Whitelegge JP, Damoiseaux R, Torres JZ, DUSP12 promotes cell cycle progression and protects cells from ZNF622 mediated apoptosis. Cell Death Dis, 17(1):(2026) [pubmed]

submitter keyword: DUSP12, proteomics, ZNF622, phosphorylation, phosphoproteomics

Mehdi Bouhaddou
contact affiliation	UCLA
contact email	bouhaddoulab@gmail.com
lab head
Mehdi Bouhaddou
contact affiliation	UCLA
contact email	bouhaddoulab@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072946
     1. Label: PRIDE project
     2. Name: DUSP12 promotes cell cycle progression and protects cells from cells from ZNF622 mediated apoptosis