PXD072887

PXD072887 is an original dataset announced via ProteomeXchange.

Title	Tenebrio molitor protein mix after growth on Cupravidus necator biomass and oat flakes
Description	This study assesses the crude protein mix from whole mealworms as an alternative to the traditionally used chlorinated organic solvents for the extraction of the greenest biodegradable biopolymers emerging on the market - polyhydroxyalkanoates. The biopolymers recovered were of comparable quality to those recovered by CHCl3 extraction, as determined by GPC and NMR. Crude protein mix exceeded the efficiency of commercial enzymes at up to 10-fold lower enzyme load. Therefore, this approach is not only a safer, non-toxic alternative to traditional organic solvents, but also an economically viable option. A proteomic study of the mealworm proteins after growth on 100% bran (TM mix control) and on 100% Cupravidus necator biomass (TM mix 2) was performed to identify enzymes responsible for the recovery effect.
HostingRepository	PRIDE
AnnounceDate	2026-05-04
AnnouncementXML	Submission_2026-05-03_23:53:05.463.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Romanos Siaperas
SpeciesList	scientific name: Tenebrio molitor; NCBI TaxID: NEWT:7067;
ModificationList	iodoacetic acid derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-10 14:33:39	ID requested
⏵ 1	2026-05-03 23:53:06	announced

Bogojevic SS, Pantelic B, Ponjavic M, Ilic-Tomic T, Milivojevic D, Guzik M, Rychwalski M, Siaperas R, Topakas E, Carvalheira M, Freitas F, Conc, ó, rdio-Reis P, Nikodinovic-Runic J, Tenebrio molitor-Derived Enzyme Systems Enable Solvent-Reduced Recovery of Intracellular Polyhydroxyalkanoates. Microb Biotechnol, 19(5):e70360(2026) [pubmed]

10.1111/1751-7915.70360;

submitter keyword: Tenebrio molitor, DIA-NN, mealworm, biopolymers,polyhydroxyalkanoates

Jasmina Nikodinovic-Runic
contact affiliation	Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, 11000, Belgrade, Republic of Serbia
contact email	jasmina.nikodinovic@imgge.bg.ac.rs
lab head
Romanos Siaperas
contact affiliation	Industrial Biotechnology & Biocatalysis Group, Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, Athens, Greece
contact email	roman_siap@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD072887

PRIDE project URI

• PRIDE
  1. PXD072887
     1. Label: PRIDE project
     2. Name: Tenebrio molitor protein mix after growth on Cupravidus necator biomass and oat flakes