PXD072844

PXD072844 is an original dataset announced via ProteomeXchange.

Title	A deep origin of the RAF–SnRK2–PP2C functional module with distinctive ancestral features
Description	The SNF1-related protein kinase 2 (SnRK2) pathway is central to abiotic stress signaling in land plants; however, its evolutionary origin and ancestral functions remain poorly understood. Here, we identify and characterize a functional SnRK2 signaling module in the unicellular green alga Chlamydomonas reinhardtii. We demonstrate that the algal isoform SnRK2.7 is essential for osmotic stress tolerance, providing a pre- abscisic acid (ABA) model system for characterizing SnRK2 signaling. Functional analysis reveals that SnRK2.7 exhibits key regulatory elements conserved in land plant SnRK2s, but also displays lineage-specific features: it is required for general cellular viability in algae and localizes to the contractile vacuole—an osmoregulatory organelle lost during streptophyte evolution. Notably, SnRK2.7 phosphorylates PP2C3, a clade A protein phosphatase, revealing an ancestral regulatory logic distinct from that in angiosperms, where PP2Cs functions upstream, dephosphorylating SnRK2 proteins. Together with MAPKKK3, a B1/B3-RAF kinase, these proteins form an evolutionarily ancient SnRK2 signaling module. Our findings reveal that a sophisticated SnRK2 pathway predates both the evolution of ABA signaling and plant terrestrialization, implying key regulatory innovations that emerged during chlorophyte-streptophyte divergence.
HostingRepository	PRIDE
AnnounceDate	2026-07-02
AnnouncementXML	Submission_2026-07-02_02:01:05.331.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	GKN JK
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: NEWT:3055;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-09 04:50:38	ID requested
⏵ 1	2026-07-02 02:01:06	announced

10.1111/NPH.71438;

submitter keyword: RAF kinase, cell survival, clade A PP2C,SnRK2, Chlamydomonas reinhardtii

Xiaobo Li
contact affiliation	Associate professer
contact email	lixiaobo@westlake.edu.cn
lab head
GKN JK
contact affiliation	Westlake University
contact email	guokangning@westlake.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD072844

PRIDE project URI

• PRIDE
  1. PXD072844
     1. Label: PRIDE project
     2. Name: A deep origin of the RAF–SnRK2–PP2C functional module with distinctive ancestral features