PXD072831

PXD072831 is an original dataset announced via ProteomeXchange.

Title	Acute e‑cigarette aerosol condensate exposure alters the transcriptome and proteome of human bronchial epithelial BEAS‑2B cells
Description	Electronic cigarettes (e‑cigarettes) generate aerosols by heating e‑liquids composed of propylene glycol, vegetable glycerin, nicotine, and flavoring agents. Although considered safer than conventional cigarettes, thermal decomposition of e‑liquids produces reactive carbonyls such as formaldehyde and acetaldehyde, and device hardware contributes metals and silicates. The health consequences of exposure remain incompletely understood, with evidence pointing to oxidative stress, mitochondrial dysfunction, and altered cellular responses. In this study, we investigated the acute effects of sub‑cytotoxic concentrations of e‑cigarette aerosol condensates on the human bronchial epithelial cell line BEAS‑2B. Condensates were generated from carrier liquids alone and from liquids containing nicotine and flavoring agents. We applied integrated transcriptomic and proteomic profiling, including assessment of protein modifications. Our results show that even short‑term exposure alters RNA and protein expression, disrupts protein translation and mitochondrial function, and is accompanied by irreversible protein modifications.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:23:42.566.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD072831
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katarina Smiljanic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-08 16:36:45	ID requested
⏵ 1	2026-04-05 16:23:43	announced

10.3390/cells15060525;

10.6019/PXD072831;

Trifunovic S, Ku, š, i, ć, -Ti, š, ma J, Smiljani, ć K, Divac Rankov A, Dini, ć J, Ljuji, ć M, Acute E-Cigarette Aerosol Condensate Exposure Disrupts the Transcriptome and Proteome Profiles of Human Bronchial Epithelial BEAS-2B Cells. Cells, 15(6):(2026) [pubmed]

submitter keyword: LC-MS/MS, BEAS-2B cells, electronic cigarettes, nicotine, proteome

Tanja Cirkovic Velickovic
contact affiliation	University of Belgrade - Faculty of Chemistry
contact email	tcirkov@chem.bg.ac.rs
lab head
Katarina Smiljanic
contact affiliation	Senior Research Associate at University of Belgrade-Faculty of Chemistry, Center of Excellence for Molecular Food Sciences
contact email	katarinas@chem.bg.ac.rs
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072831
     1. Label: PRIDE project
     2. Name: Acute e‑cigarette aerosol condensate exposure alters the transcriptome and proteome of human bronchial epithelial BEAS‑2B cells