PXD072780

PXD072780 is an original dataset announced via ProteomeXchange.

Title	Structural Diversity of Lipid A Modulates Neutrophil Proteome and Secretome Responses
Description	We applied quantitative high-resolution proteomics to systematically evaluate how the human PMN proteome is shaped in response to two LPS preparations that differ primarily in the number of acyl chains associated with their lipid A (E. coli and F. tularensis LPS). To capture both stimulus-specific effects and response kinetics, PMNs were analyzed at multiple time points, allowing assessment of treatment-dependent differences and proteomic changes over time within each condition. We characterized the PMN secretome following exposure to these agents, enabling the comparison of process between intracellular proteomic and protein release. Together, this integrated cellular proteome and secretome approach provides a comprehensive framework to define how structurally distinct LPS molecules modulate PMN functional programs over time. While PMN responses to LPS have been extensively investigated, their temporal proteomic and secretory landscapes in primary human PMNs, particularly in response to LPS with divergent lipid A architectures, remain incompletely characterized.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_21:33:33.040.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Nita-Lazar
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Eclipse; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-07 16:24:08	ID requested
⏵ 1	2026-04-26 21:33:34	announced

10.1016/j.jprot.2026.105643;

Pittaluga Villarreal JR, Kim D, Yoon SH, Bhushan V, Issara-Amphorn J, Pederson J, Manes NP, Nita-Lazar A, Structural diversity of lipid A modulates neutrophil proteome and secretome responses. J Proteomics, 328():105643(2026) [pubmed]

submitter keyword: LPS, Francisella tularensis, Escherichia coli,Neutrophils, Secretome

Aleksandra Nita-Lazar
contact affiliation	Functional Cellular Networks Section, Laboratory of Immune System Biology, NIAID, NIH
contact email	nitalazarau@niaid.nih.gov
lab head
Aleksandra Nita-Lazar
contact affiliation	NIAID
contact email	nitalazarau@niaid.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD072780

PRIDE project URI

• PRIDE
  1. PXD072780
     1. Label: PRIDE project
     2. Name: Structural Diversity of Lipid A Modulates Neutrophil Proteome and Secretome Responses