PXD072771

PXD072771 is an original dataset announced via ProteomeXchange.

Title	Blood-brain barrier disrupting stimuli induce production of extracellular vesicles with distinct protein cargoes and functionality
Description	The blood-brain barrier (BBB) protects the brain from substances in the circulation; yet, this barrier can be breached. Systemic lupus erythematosus is a disease in which over 50% of patients experience manifestations of neuropsychiatric lupus (NPSLE), with many displaying BBB dysfunction. High levels of TNFα, IL-1b, C5a, and epinephrine, which have all been shown to cause BBB permeability, may be present in the serum of SLE patients. Brain microvascular endothelial cells (BMVECs) are a major structural element of the BBB. We asked whether BMVECs stimulated with barrier-disrupting factors release extracellular vesicles (EVs) which might participate in autocrine signaling or might have utility as diagnostic biomarkers of BBB permeability. We analyzed proteins in EVs secreted by unstimulated and stimulated BMVECs by mass spectrometry and used secreted EVs for further BMVEC stimulation. We subjected BMVECs to bulk RNA-seq to identify response signatures to the initial stimulus and to secondary stimulation with EVs. We show that there are agent-specific EV-associated protein profiles. EVs from BMVECs subjected to BBB-breaching stimuli alter the BMVEC transcriptional program, representing a potential feed-forward mechanism. Finally, we suggest that the proteins associated with EVs might be markers of BBB disruption.
HostingRepository	PRIDE
AnnounceDate	2026-02-02
AnnouncementXML	Submission_2026-02-02_14:49:26.841.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	henrik molina
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-07 13:15:57	ID requested
⏵ 1	2026-02-02 14:49:27	announced

Dataset with its publication pending

submitter keyword: Blood-brain, cargo, protein, extracellular, barrier, vesicles

Betty Diamond
contact affiliation	Institute of Molecular Medicine The Center for Autoimmune, Musculoskeletal and Musculoskeletal Disease Feinstein Institutes for Medical Research Northwell Health 350 Community Drive Manhasset, NY 11030
contact email	BDiamond@northwell.edu
lab head
henrik molina
contact affiliation	THE ROCKEFELLER UNIVERSITY
contact email	henrik.molina@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD072771

PRIDE project URI

• PRIDE
  1. PXD072771
     1. Label: PRIDE project
     2. Name: Blood-brain barrier disrupting stimuli induce production of extracellular vesicles with distinct protein cargoes and functionality