PXD072749

PXD072749 is an original dataset announced via ProteomeXchange.

Title	Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes
Description	Ubiquitination is the key eukaryotic post-translational modification that governs protein degradation, localisation, and activity which is mediated by a concerted enzyme cascade. The largest superfamily of these enzymes include the Cullin-RING-Ligase (CRL) complexes. Plasmodium falciparum, the causative agent of the most severe form of malaria in humans, encodes the critical proteins required for ubiquitination, but we do not yet understand the function of this pathway. Here the P. falciparum CRL complexes were characterised to reveal an essential but minimal repertoire controlled by two Cullin scaffolds. A PfCullin1-linked CRL complex, recruiting a single substrate receptor, was identified as being required for parasite inner-membrane biogenesis and DNA replication. A second CRL complex functioning through a PfCullin4 scaffold was identified that utilised a previously unidentified adaptor protein and receptors to support DNA replication. These results show that the P. falciparum CRL complexes are essential in both nuclear maintenance and membrane integrity.
HostingRepository	PRIDE
AnnounceDate	2026-01-09
AnnouncementXML	Submission_2026-01-09_01:51:46.988.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Dagley
SpeciesList	scientific name: Plasmodium falciparum (isolate 3D7); NCBI TaxID: NEWT:36329;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-07 02:41:54	ID requested
⏵ 1	2026-01-09 01:51:47	announced

10.1038/s41598-025-05342-0;

10.1038/S41598-025-05342-0;

Marapana D, Cobbold SA, Pasternak M, Shami GJ, Ralph SA, Lopaticki S, Yousef J, Vaibhav V, Dagley LF, Komander D, Cowman AF, Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes. Sci Rep, 15(1):21359(2025) [pubmed]

submitter keyword: None

Alan Cowman
contact affiliation	The Walter and Eliza Hall institute of Medical Research, Department of Medical Biology, Faculty of Medicine, Dentistry and Health Sciences, The University of Melbourne
contact email	cowman@wehi.edu.au
lab head
Laura Dagley
contact affiliation	WEHI
contact email	dagley.l@wehi.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD072749

PRIDE project URI

• PRIDE
  1. PXD072749
     1. Label: PRIDE project
     2. Name: Functional characterisation of components in two Plasmodium falciparum Cullin-RING-Ligase complexes