⮝ Full datasets listing
PXD072742
PXD072742 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | pyroptosis, BBB disruption, bacterial meningitis, endothelial cell, microglia |
| Description | Blood-brain barrier (BBB) is a highly specialized membrane that maintains brain homeostasis. The breakdown of the BBB is associated with various neuro-inflammatory diseases. The mechanisms of inflammatory BBB disruption and inflammation transmission in the neuroinflammatory microenvironment remain largely unknown. Here, we showed that brain endothelial cells (ECs) prominently responded to neonatal meningitis Escherichia coli (NMEC) challenge and underwent GSDMD-dependent pyroptosis. By using spatialtemporal single-cell transcriptomic sequencing and proteomics sequencing of mouse brain after NMEC infection, we found that Gbp5 was required for brain EC pyroptosis during inflammatory BBB disruption. Furthermore, we identified a homeostasis-inflammation state transition both in EC and microglia (MG) during pyroptosis. Pyroptotic EC can induce MG pyroptosis via Angptl4-Sdc4 signaling. This interaction leads to a model of “pyroptosis cascade” between brain EC and MG in the central nervous system (CNS). The EC-MG py |
| HostingRepository | iProX |
| AnnounceDate | 2026-01-07 |
| AnnouncementXML | Submission_2026-01-06_23:26:38.123.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhiyue Wu |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-06 23:26:07 | ID requested | |
| ⏵ 1 | 2026-01-06 23:26:38 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: pyroptosis, BBB disruption, bacterial meningitis, endothelial cell, microglia |
Contact List
| Haiqing Xiong | |
|---|---|
| contact affiliation | State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital |
| contact email | xionghaiqing@ihcams.com |
| lab head | |
| Zhiyue Wu | |
| contact affiliation | Tianjin Medical University |
| contact email | zhiyuewu@tmu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
