PXD072730

PXD072730 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of Streptococcus pneumoniae isolates in adapted medium
Description	Here, we examined how pneumococci adapt to the upper respiratory tract conditions, and how this affects interactions with the host. We established the intranasal microenvironment including metal ion and monosaccharide concentrations to create in vivo-mimicking medium to study pneumococcal adaptation to the human host. While growth of pneumococci in this medium was reduced, no major differences were found in capsular polysaccharide structure or thickness. Proteome analysis revealed a shift to galactose as carbohydrate source and decreased levels of fatty acid biosynthesis proteins and pneumolysin. Exo-metabolome analysis revealed accumulation of glycerophosphocholine leading to inhibited CRP and IgM binding to pneumococci. Pneumococci grown in in vivo-mimicking medium were more capable colonisers of differentiated primary epithelial cell layers and induced less epithelial cytokine release. Together these data show how pneumococci adapt to the nutrient-limited respiratory environment and modulate cells and humoral responses to facilitate persistent colonisation.
HostingRepository	PRIDE
AnnounceDate	2026-08-14
AnnouncementXML	Submission_2026-08-14_03:27:54.025.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kristin Surmann
SpeciesList	scientific name: Streptococcus pneumoniae; NCBI TaxID: NEWT:1313;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-06 19:10:23	ID requested
⏵ 1	2026-08-14 03:27:54	announced

Dataset with its publication pending

submitter keyword: BHN418, mucin, BHN100,Streptococcus pneumoniae

Uwe Völker
contact affiliation	University Medicine Greifswald
contact email	voelker@uni-greifswald.de
lab head
Kristin Surmann
contact affiliation	University Medicine Greifswald
contact email	surmannk@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD072730

PRIDE project URI

• PRIDE
  1. PXD072730
     1. Label: PRIDE project
     2. Name: Proteome analysis of Streptococcus pneumoniae isolates in adapted medium