PXD072727

PXD072727 is an original dataset announced via ProteomeXchange.

Title	Screening and validation of biomarkers for radiation pneumonitis based on targeted plasma proteomics
Description	The absence of reliable biomarkers for predicting the risk and severity of radiation pneumonitis (RP) prior to radiotherapy represents a major clinical challenge, as current tools rely on delayed imaging and lack specificity. The molecular mechanisms that determine why only a subset of patients progress to severe lung injury remain largely unknown. To address this, we conducted a preliminary prospective plasma proteomic profiling in patients with non-small cell lung cancer (NSCLC) before thoracic radiotherapy. Using high-resolution data-independent acquisition (DIA) mass spectrometry, we screened for circulating proteins associated with subsequent RP development. Through this approach and cross-species validation in a murine model of radiation-induced lung injury, we preliminarily identified THEMIS2 as a potential central predictive hub protein. Its baseline plasma level showed a strong correlation with subsequent clinical severity. Critically, while Themis2 was upregulated in irradiated lung tissue, its expression was significantly lower in non-surviving animals compared to survivors. This dual expression pattern supports a preliminary "compensation-exhaustion" hypothesis: elevated baseline THEMIS2 may indicate a pre-sensitized, compensatory immune state potentially associated with higher risk, whereas the failure to sustain this response post-irradiation appears correlated with fatal outcome. Collectively, our preliminary findings suggest THEMIS2 as a novel candidate biomarker that may link pre-treatment risk assessment to post-injury prognosis, implying a potential key mechanism in RP pathogenesis and providing a preliminary molecular basis for early intervention strategies. These findings require validation in larger cohorts.
HostingRepository	iProX
AnnounceDate	2026-01-07
AnnouncementXML	Submission_2026-01-06_17:49:53.551.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Min Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-06 17:49:34	ID requested
⏵ 1	2026-01-06 17:49:54	announced

Dataset with its publication pending

submitter keyword: radiation pneumonitis, plasma proteomics, biomarker, THEMIS2, non-small cell lung cancer

Ping Lei
contact affiliation	Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University)
contact email	2506421373@qq.com
lab head
Min Zhang
contact affiliation	Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University)
contact email	654916662@qq.com
dataset submitter

iProX dataset URI