PXD072710

PXD072710 is an original dataset announced via ProteomeXchange.

Title	Effects of human immunoglobulins on Cryptococcus neoformans morphology and proteome
Description	Cryptococcus neoformans (Cn) is the main cause of fungal meningitis (CM) in people living with HIV. Perturbations in levels of normal immunoglobulin (Ig) levels are observed in these individuals, but their association with CM pathogenesis is unclear. Here, we investigated the physical and biological effects of normal human immunoglobulins (not elicited by known cryptococcal infection), IgM, IgG, and IgA on Cn (strain H99). Each isotype affected the growth, surface morphology, and proteome of Cn. However, IgA had the most prominent effect. It induced growth inhibition after 24 h of co-culture with Cn, altered the structural organization of capsular fibers, and significantly reduced protein synthesis and proteins associated with intracellular glucuronoxylomannan (GXM) synthesis, such as those mediating transport of sugar precursors to Golgi and the cyclic AMP (cAMP) pathway. Together with prior data showing an association between reduced plasma IgA and HIV-associated CM, our findings suggest that the influence on human IgA on Cn pathogenesis warrants further investigation.
HostingRepository	PRIDE
AnnounceDate	2026-01-26
AnnouncementXML	Submission_2026-01-26_11:59:56.867.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlon D M Santos
SpeciesList	scientific name: Cryptococcus neoformans; NCBI TaxID: NEWT:5207;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-06 07:15:54	ID requested
⏵ 1	2026-01-26 11:59:57	announced

Dataset with its publication pending

submitter keyword: IgM, cryptococcal capsule,Cryptococcus neoformans, human immunoglobulins, IgG, HIV, cryptococcal proteome., and IgA, glucuronoxylomannan

Taiane N de Souza
contact affiliation	Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA
contact email	taianensouza@gmail.com
lab head
Marlon D M Santos
contact affiliation	Computational Mass Spectrometry & Proteomics Group - Fiocruz
contact email	marlondms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072710
     1. Label: PRIDE project
     2. Name: Effects of human immunoglobulins on Cryptococcus neoformans morphology and proteome