⮝ Full datasets listing
PXD072700
PXD072700 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ancestry-Linked IL-10 Signaling and Macrophage Activation Modulate Fibroblast Responses to Oxidative Stress in a PEG-Based Microphysiological System |
| Description | Fibroblast responses to injury are shaped by cytokine signaling and macrophage activation, yet the extent to which these pathways vary across individuals, and how ancestry associated immune differences influence fibrosis risk, remains poorly understood. Here, we developed a poly(ethylene glycol) (PEG)-based hydrogel microphysiological system to model fibroblast-macrophage interactions following oxidative stress and integrate donor-specific immune signals using matched macrophages and serum. Circulating monocytes from individuals of self-reported African American ancestry exhibited higher expression of CCL4 and increased serum IL10, whereas those from European ancestry donors showed elevated OXER1 expression. Within the hydrogel, oxidative stress reduced fibroblast number while inducing Ki67 and p16. Exogenous TGFbeta1 enhanced fibroblast survival and collagen 3 production but did not independently increase alpha smooth muscle actin (alphaSMA). Anti-inflammatory macrophages promoted fibroblast activation through mechanisms resistant to TGFbeta receptor inhibition. Incorporating donor-derived macrophages and serum revealed that cultures from individuals of European ancestry demonstrated higher fibroblast alphaSMA and p16 expression, despite lower systemic IL10 levels. Pharmacologic inhibition of IL10 further increased alphaSMA, particularly in European ancestry derived cultures, identifying IL10 as a key protective signal limiting fibroblast activation. This hydrogel system provides a platform for dissecting inter-individual immune variation and identifying mechanisms underlying ancestry associated fibrosis risk. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-06 |
| AnnouncementXML | Submission_2026-01-06_22:57:35.326.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Paul Parodi |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; TMTpro; Oxidation; Deamidated; Acetyl; Met-loss; Met-loss+Acetyl |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-06 01:07:07 | ID requested | |
| ⏵ 1 | 2026-01-06 22:57:35 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: sera, DatasetType:Proteomics |
Contact List
| Erika Taylor Moore | |
|---|---|
| contact affiliation | University of Maryland |
| contact email | emt@umd.edu |
| lab head | |
| Paul Parodi | |
| contact affiliation | University of Maryland |
| contact email | parodipaul@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v11/MSV000100385/ |
