PXD072661

PXD072661 is an original dataset announced via ProteomeXchange.

Title	Efficient globin production during terminal erythropoiesis depends on the cooperative action of TENT5C poly(A) polymerase and LARP4B in mice
Description	To identify erythroid-specific TENT5C interactors, we employed a modified TurboID proximity biotinylation assay followed by MS/MS analysis of fetal liver erythroblasts differentiated ex vivo. We took advantage of Tent5c-EGFP knock-in mouse line and employed lentiviral vector expressing nanobody anti-EGFP fused with TurboID biotinylase. By introducing this construct to either WT or Tent5c-EGFP erythroblasts, we were able to quantitatively determine the specificity of enriched proteins found in proximity to TENT5C-EGFP compared to the background found in the WT. This approach does not require the introduction of exogenous biotinylase-tagged TENT5C, which expression would be out of place in the earliest stages of erythropoiesis. Importantly, it was designed for the knock-in model in which the expression timing for TENT5C is in accord with the developmental timeline of erythroblasts. Hence, we expected the enriched proximal proteins to be physiologically relevant interactors of TENT5C.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-29_05:28:01.620.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD072661
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrzej Dziembowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2026-01-05 11:11:10	ID requested
⏵ 1	2026-06-29 05:28:02	announced

10.6019/PXD072661;

submitter keyword: cytoplasmic polyadenylation, TENT5C,erythropoiesis, interactome

Andrzej Dziembowski
contact affiliation	Laboratory of RNA Biology, International Institute of Molecular and Cellular Biology
contact email	adziembowski@iimcb.gov.pl
lab head
Andrzej Dziembowski
contact affiliation	International Institute of Molecular and Cell Biology in Warsaw, Poland
contact email	adziembowski@iimcb.gov.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD072661
     1. Label: PRIDE project
     2. Name: Efficient globin production during terminal erythropoiesis depends on the cooperative action of TENT5C poly(A) polymerase and LARP4B in mice