⮝ Full datasets listing
PXD072639
PXD072639 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | CRISPR-Cas9 Screening Identifies CDK2 as a Critical Host Factor Enhancing Zika Virus RdRp Activity and Immune Evasion |
| Description | Zika virus (ZIKV) is a global health threat that can cause severe neurological defects, but specific antiviral therapies are lacking. Through a kinase-focused CRISPR-Cas9 screen, we identified cyclin-dependent kinase 2 (CDK2) as an essential host factor for ZIKV infection. We demonstrate that CDK2 directly binds to and phosphorylates the viral RNA polymerase NS5, enhancing its RNA synthesis activity to drive viral replication. In human cells, this phosphorylation also potentiates NS5-mediated degradation of STAT2 and suppression of type I interferon signaling, representing a synergistic immune-evasion mechanism. Pharmacological inhibition of CDK2 with clinical-stage compounds, SNS-032 and Fadraciclib, effectively limited ZIKV replication in human cells, brain organoids, and immunocompromised mice. These inhibitors also restricted Dengue virus replication, indicating that targeting host CDK2 could be a promising strategy against other flaviviruses. Our work unveils CDK2 as a critical host kinase co-opted by ZIKV and establishes its inhibition as a potential host-directed antiviral approach. |
| HostingRepository | iProX |
| AnnounceDate | 2026-01-04 |
| AnnouncementXML | Submission_2026-01-05_01:04:21.200.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Ruixia Tang |
| SpeciesList | scientific name: Zika virus; NCBI TaxID: 64320; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-01-05 01:04:06 | ID requested | |
| ⏵ 1 | 2026-01-05 01:04:21 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: ZIKV, CRISPR-CAS9, CDK2, RdRp, Immune evasion |
Contact List
| Qiangming Sun | |
|---|---|
| contact affiliation | Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College |
| contact email | qsun@imbcams.com.cn |
| lab head | |
| Ruixia Tang | |
| contact affiliation | Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College |
| contact email | trx7927@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
